公开(公告)号：EP0950718A1

公开(公告)日：1999-10-20

申请号：EP98931022.2

申请日：1998-07-09

申请人： DAI NIPPON PRINTING CO., LTD. ,  Karube, Isao

发明人： KARUBE, Isao ,  SAWATA, Shinya ,  NAGATA, Ryohei, Dai Nippon Printing Co., Ltd.

IPC分类号： C12Q1/68 ,  G01N21/27

CPC分类号： C12Q1/6825 ,  C12Q1/6837 ,  Y02A50/52 ,  C12Q2525/107

摘要： A method for detecting a target nucleotide sequence by a hybridization procedure with an extremely improved detecting sensitivity. This method involves the step of hybridizing the target nucleotide sequence with a PNA which is complementary to the whole target nucleotide sequence or a part thereof and the step of measuring the extent of the hybridization.

摘要翻译： 一种以极高的检测灵敏度通过杂交程序检测靶核苷酸序列的方法。 该方法包括使靶核苷酸序列与与全部靶核苷酸序列或其一部分互补的PNA杂交的步骤和测定杂交程度的步骤。

公开(公告)号：EP0915174A1

公开(公告)日：1999-05-12

申请号：EP98919518.5

申请日：1998-05-08

申请人： Karube, Isao ,  DAI NIPPON PRINTING CO., LTD.

发明人： KARUBE, Isao ,  SAWATA, Shinya ,  NAGATA, Ryohei Dai Nippon Printing Co., Ltd.

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6832 ,  C12Q1/686 ,  C12Q2531/107 ,  C12Q2565/501

摘要： An object of the present invention is to provide a method for detecting a target nucleotide sequence using a complementary nucleotide sequence that has an excellent sensitivity of detection. The method comprises the steps of converting the target nucleotide sequence to a partially double-stranded nucleotide sequence which is double-stranded at one part and single-stranded in the remaining part and detecting said partially double stranded nucleotide sequence using a nucleotide sequence that is complementary to the target nucleotide sequence.

摘要翻译： 本发明的目的是提供使用检测灵敏度优异的互补核苷酸序列检测靶核苷酸序列的方法。 该方法包括以下步骤：将靶核苷酸序列转化为部分双链核苷酸序列，其一部分为双链，其余部分为单链，并使用互补的核苷酸序列检测所述部分双链核苷酸序列 到目标核苷酸序列。

公开(公告)号：EP0915174B1

公开(公告)日：2007-10-10

申请号：EP98919518.5

申请日：1998-05-08

申请人： Karube, Isao ,  DAI NIPPON PRINTING CO., LTD.

发明人： KARUBE, Isao ,  SAWATA, Shinya ,  NAGATA, Ryohei Dai Nippon Printing Co., Ltd.

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6832 ,  C12Q1/686 ,  C12Q2531/107 ,  C12Q2565/501

摘要： A method for detecting a target nucleotide sequence at a high detection sensitivity with the use of a complementary nucleotide sequence. This method involves the step of converting the target nucleotide sequence into a partly double-stranded nucleotide sequence which is double-stranded in some parts but single-stranded in the others; and the step of detecting the partly double-stranded nucleotide sequence as described above with the use of the nucleotide sequence complementary to the target nucleotide sequence.