公开(公告)号：EP1143251A4

公开(公告)日：2002-03-20

申请号：EP99900171

申请日：1999-01-12

申请人： EBARA CORP ,  KARUBE ISAO

发明人： KARUBE ISAO ,  NOMURA YOKO

IPC分类号： G01N33/543

CPC分类号： G01N33/54373

摘要： A sensor chip for surface plasmon resonance which has a thin metal film, an antibody against a substance with the steroid skeleton such as a steroid hormone, a sex hormone or an environmental hormone (excluding triazine compounds), and a fixation layer for fixing the antibody to the thin metal film; and a method for detecting an environmental hormone which involves the step of exposing the antibody in the above-mentioned sensor chip to a sample, the step of irradiating the thin metal film with light and the step of determining the intensity of the light reflected by the thin metal film. This method achieves a high sensitivity and an excellent selectivity.

公开(公告)号：EP1063287A1

公开(公告)日：2000-12-27

申请号：EP99939150.1

申请日：1999-03-12

申请人： Karube, Isao ,  Saitoh, Takashi ,  Center for Advanced Science and Technology Incubation, Ltd

发明人： Karube, Isao ,  Saitoh, Takashi ,  Center for Advanced Science and Technology Incubation, Ltd

IPC分类号： C12M1/00 ,  C12N5/06

CPC分类号： C12N15/89 ,  C12M35/00 ,  C12N13/00

摘要： A technique for controlling membrane denaturation reactions other than physical shear force was developed. For example, the present invention provides, a method for causing membrane disruption at a specific site by reacting a stimulus such as light with a compound that is activated by the stimulus, where the reaction occurs on a membrane such as a biomembrane. It also provides a membrane structure such as cells in which a specific site has been disrupted, which are obtained by the present method. Introduction of substances such as genes also became possible by using this membrane structure. Further provided is a membrane-destroying member for disrupting a membrane at a specific site. Thus, the present invention enabled, for example, easy membrane penetration using components constituting microelectrodes, micromanipulators, and microinjectors, which were conventionally hardly usable in penetrating cell membranes.

摘要翻译： 开发了除物理剪切力之外的用于控制膜变性反应的技术。 例如，本发明提供了通过使诸如光的刺激与由刺激激活的化合物反应而使特定部位的膜破裂的方法，其中反应发生在诸如生物膜的膜上。 还提供了通过本方法获得的膜结构，例如其中特定部位已被破坏的细胞。 通过使用这种膜结构也可以引入基因等物质。 还提供了用于破坏特定部位的膜的膜破坏部件。 因此，本发明能够例如使用通常几乎不能用于穿透细胞膜的微电极，微操纵器和微注射器的构成的薄膜穿透。

公开(公告)号：EP0767376A2

公开(公告)日：1997-04-09

申请号：EP96307270.7

申请日：1996-10-04

申请人： Karube, Isao ,  TOKUYAMA CORPORATION ,  A & T Corporation

发明人： Karube, Isao ,  Iwata, Keisuke ,  Yoshimura, Yoshimichi

IPC分类号： G01N33/543

CPC分类号： G01N33/5438

摘要： A method for detecting or measuring an immunologically reactive substance through an immunological reaction with a reagent which is an antigen, an antibody, a carrier reagent sensitized by an antigen, or a carrier reagent sensitized by an antibody, wherein an immunological reaction product is produced in an aqueous reaction system by applying to the aqueous reaction system in which the reagent and the immunologically reactive substance are present a small voltage (preferably insufficient to cause electrolysis of the system) without substantially forming a pearl chain derived from the reagent, i.e. when the reagent includes a carrier three or more particles of carrier reagent are not arranged adjacent to one another in the form of a straight chain.

摘要翻译： 一种通过免疫反应来检测或测定免疫反应性物质的方法，所述试剂是抗原，抗体，由抗原敏化的载体试剂或由抗体敏化的载体试剂，其中产生免疫反应产物 通过向其中存在试剂和免疫反应性物质的水性反应体系施加小的电压（优选不足以引起系统的电解）而基本上不形成来自试剂的珍珠链，即当试剂 包括载体三个或更多个载体试剂颗粒不以直链的形式彼此相邻布置。

公开(公告)号：EP0284518B1

公开(公告)日：1992-10-07

申请号：EP88400742.8

申请日：1988-03-25

申请人： Karube, Isao ,  FUJITSU LIMITED

发明人： Karube, Isao ,  Suzuki, Hiroaki

IPC分类号： G01N27/48 ,  G01N27/30 ,  C12M1/40 ,  G01N27/416

CPC分类号： G01N27/404 ,  C12Q1/005 ,  Y10S435/817 ,  Y10T29/417

公开(公告)号：EP0945721B1

公开(公告)日：2004-11-17

申请号：EP99105079.0

申请日：1999-03-24

申请人： DAI NIPPON PRINTING CO., LTD. ,  Karube, Isao

发明人： Nakamura, Runa ,  Nakamura, Hiroyuki ,  Nagata, Ryohei ,  Karube, Isao ,  Muguruma, Hitoshi

IPC分类号： G01N21/55

CPC分类号： G01N21/553

公开(公告)号：EP0811838A4

公开(公告)日：2000-05-17

申请号：EP96903204

申请日：1996-02-21

申请人： AKEBONO BRAKE IND ,  AKEBONO RESEARCH AND ,  KARUBE ISAO

发明人： KARUBE ISAO ,  YANO KIYOKO ,  YOSHIDA NOBUYUKI ,  MORITA TAKASHI

IPC分类号： G01N27/327 ,  C12Q1/00 ,  C12Q1/02 ,  G01N27/416

CPC分类号： C12Q1/001 ,  C12Q1/004

摘要： A microbial electrode comprising a substrate (3) of insulator material, an electric conductor (2) fixed to the substrate, and a thin film (1) containing microbial cells and adhered to the conductor; and a microbial sensor (20) comprising the microbial electrode and an electric conductor (11) as a counter electrode fixed to that surface of the substrate which is opposite to the surface to which the microbial cells have been adhered.

公开(公告)号：EP0945721A3

公开(公告)日：2000-01-26

申请号：EP99105079.0

申请日：1999-03-24

申请人： DAI NIPPON PRINTING CO., LTD. ,  Karube, Isao

发明人： Nakamura, Runa ,  Nakamura, Hiroyuki ,  Nagata, Ryohei ,  Karube, Isao ,  Muguruma, Hitoshi

IPC分类号： G01N21/55

CPC分类号： G01N21/553

摘要： An objective of the present invention is to provide a measuring chip for a surface plasmon resonance sensor that can detect a small amount of target substances in high sensitivity. The present invention provides a measuring chip for a surface plasmon resonance sensor comprising a metal layer, one or more plasma polymerization layers formed on said metal layer, and a biologically active substance immobilized on the surface of said plasma polymerization layer.

公开(公告)号：EP0767376A3

公开(公告)日：1998-08-12

申请号：EP96307270

申请日：1996-10-04

申请人： KARUBE ISAO ,  TOKUYAMA CORP ,  A & T CORP

发明人： KARUBE ISAO ,  IWATA KEISUKE ,  YOSHIMURA YOSHIMICHI

IPC分类号： G01N33/543

CPC分类号： G01N33/5438

摘要： A method for detecting or measuring an immunologically reactive substance through an immunological reaction with a reagent which is an antigen, an antibody, a carrier reagent sensitized by an antigen, or a carrier reagent sensitized by an antibody, wherein an immunological reaction product is produced in an aqueous reaction system by applying to the aqueous reaction system in which the reagent and the immunologically reactive substance are present a small voltage (preferably insufficient to cause electrolysis of the system) without substantially forming a pearl chain derived from the reagent, i.e. when the reagent includes a carrier three or more particles of carrier reagent are not arranged adjacent to one another in the form of a straight chain.

公开(公告)号：EP0811838A1

公开(公告)日：1997-12-10

申请号：EP96903204.4

申请日：1996-02-21

申请人： AKEBONO BRAKE INDUSTRY CO., LTD. ,  AKEBONO RESEARCH AND DEVELOPMENT CENTRE LTD. ,  Karube, Isao

发明人： KARUBE, Isao ,  YANO, Kiyoko ,  YOSHIDA, Nobuyuki Berutsuri-kowa 202 ,  MORITA, Takashi

IPC分类号： G01N27/327 ,  G01N27/416 ,  C12Q1/02

CPC分类号： C12Q1/001 ,  C12Q1/004

摘要： The microbial electrode is constructed having a substrate(3) made of an insulating material, an electric conductor(2) which is fixed on the substrate and a membrane containing microorganism cells(1) which is fixed on the conductor. The microbial sensor(20) is constructed having the microbial electrode above described and a conductor(11) to act as a counter electrode fixed on the insulating material of the surface of the substrate of the electrode which is opposite to the surface where the membrane containing microorganism cells is fixed.

摘要翻译： 微生物电极构造成具有由绝缘材料制成的基板（3），固定在基板上的电导体（2）和固定在导体上的含有微生物细胞（1）的膜。 微生物传感器（20）被构造成具有上述微生物电极和导体（11），用作固定在电极基底表面的绝缘材料上的相对电极，该绝缘材料与含有 微生物细胞是固定的。

公开(公告)号：EP0667521A1

公开(公告)日：1995-08-16

申请号：EP94925015.3

申请日：1994-08-26

申请人： AKEBONO BRAKE INDUSTRY CO., LTD. ,  AKEBONO RESEARCH AND DEVELOPMENT CENTRE LTD. ,  Karube, Isao

发明人： KARUBE, Isao ,  YANO, Kiyoko

IPC分类号： G01N27/327 ,  G01N27/416

CPC分类号： C12Q1/001 ,  G01N33/1806

摘要： An electrode made of a metal or carbon, a working electrode (20) in contact with this electrode and of a microorganic germ-containing thin film, a counter electrode (7), and a reference electrode (5) as necessary are immersed in an organism-containing solution (30), and the BOD of this solution is measured by determining the quantity of an electric current flowing between the working electrode (20) and counter-electrode (7) or reference electrode (5), applying a voltage between the electrodes by a potentiostat (6). By this method, the concentrations of organic compounds can be measured directly without using an oxygen electrode even when the quantity of dissolved oxygen is small.

摘要翻译： 将由金属或碳制成的电极，与该电极接触的工作电极（20）和含有微生物的含有有机物的薄膜，对电极（7）和必要时的参比电极（5）浸渍在 含有生物体的溶液（30），并且通过确定在工作电极（20）和对电极（7）或参考电极（5）之间流动的电流的量来测量该溶液的BOD， 电极通过恒电位仪（6）。 通过该方法，即使溶解氧量少，也可以直接测定有机化合物的浓度而不使用氧电极。