公开(公告)号：EP1560020A1

公开(公告)日：2005-08-03

申请号：EP03758985.0

申请日：2003-10-28

申请人： Katayanagi Institute ,  Yano, Kazuyoshi ,  Karube, Isao

发明人： YANO, Kazuyoshi ,  KARUBE, Isao ,  HIRATSUKA, Atsunori ,  TSAI, Shuo-Wen, 201, Koupo Arakawaoki

IPC分类号： G01N27/447 ,  B01D57/02

CPC分类号： B01D57/02 ,  G01N27/44773 ,  G01N27/44791

摘要： Transfer of fluid substances, and/or substances comprised in fluid substances, is controlled by introducing a separation medium, which prevents transfer of the fluid substances, and/or the substances comprised in the fluid substances, to an intervening cavity connecting a first cavity and a second cavity; and introducing a connection medium to replace the separation medium and thereby start substance transfer to the second cavity. Substance transfer may be readily controlled without relying on mechanical means. Based on the present invention, two-dimensional electrophoretic analysis can be readily implemented on a chip.

摘要翻译： 流体物质和/或包含在流体物质中的物质的转移通过引入防止流体物质和/或包含在流体物质中的物质转移到连接第一空腔和第二空腔的居间空腔的分离介质来控制， 第二腔体; 并且引入连接介质以替换分离介质并由此开始将物质转移到第二腔。 物质转移可以容易地控制，而不依赖于机械手段。 基于本发明，可以容易地在芯片上实施二维电泳分析。

公开(公告)号：EP1548429A1

公开(公告)日：2005-06-29

申请号：EP03799096.7

申请日：2003-09-05

申请人： Katayanagi Institute ,  National Institute of Advanced Industrial Science and Technology

发明人： HIRATSUKA, Atsunori ,  YANO, Kazuyoshi ,  KARUBE, Isao ,  TSAI, Shuo-Wen ,  YOKOYAMA, Kenji ,  KOIDE, Satoshi ,  AKIMOTO, Takuo

IPC分类号： G01N27/447

CPC分类号： B82Y30/00 ,  G01N27/447 ,  G01N27/44752

摘要： An objective of the present invention is to provide electrophoretic separation methods and devices that enable the various features of a substrate surface that comes in contact with an electrophoresis medium to be controlled.
The present invention provides methods for electrophoresing substances, which comprises the steps of:

(a) adding a substance to be analyzed to an electrophoresis medium retained in a substrate, whose surface that has come in contact with the electrophoresis medium has been coated with a polymer membrane; and
(b) adding electrophoretic pressure to the electrophoresis medium.

For example, the use of a plasma-polymerized membrane allows the formation of a membrane with homogeneous quality and thickness on the surface of an arbitrary shape. In addition, desired characteristics can be conferred on the surface through selection of monomeric substances. Protein adsorption onto micro-chips can be effectively prevented as well.

摘要翻译： 本发明的目的是提供一种电泳分离方法和装置，其能够使与基质表面接触的电泳介质的各种特征得以控制。 本发明提供了电泳物质的方法，其包括以下步骤：（a）将待分析物质加入保留在与电泳介质接触的表面的基质中的电泳介质中 涂有聚合物膜; 和（b）向电泳介质中加入电泳压力。 例如，使用等离子体聚合膜允许在任意形状的表面上形成均匀质量和厚度的膜。 此外，可以通过选择单体物质在表面上赋予所需的特性。 可以有效地防止蛋白质在微芯片上的吸附。