公开(公告)号：EP4257672A1

公开(公告)日：2023-10-11

申请号：EP21900710.1

申请日：2021-12-03

申请人： Keio University ,  JSR Corporation

发明人： SATO Toshiro ,  SUGIMOTO Shinya ,  ARAI Kazuya

IPC分类号： C12N5/071 ,  C12Q1/06

摘要： A method for producing a two-dimensional small intestinal organoid having a villus structure, the method including a step 1 of culturing a cell derived from a small intestinal epithelium in an extracellular matrix to obtain a three-dimensional small intestinal organoid, a step 2 of dispersing the three-dimensional small intestinal organoid and monolayer culturing on the extracellular matrix to obtain a two-dimensional small intestinal organoid, and a step 3 of further culturing the two-dimensional small intestinal organoid while letting a culture medium of the two-dimensional small intestinal organoid to flow so that the two-dimensional small intestinal organoid forms a villus structure.