MICROBIAL DETECTION AND QUANTIFICATION
    6.
    发明授权
    MICROBIAL DETECTION AND QUANTIFICATION 有权
    细菌证明和量化

    公开(公告)号:EP1828402B1

    公开(公告)日:2012-06-13

    申请号:EP05851252.6

    申请日:2005-10-24

    IPC分类号: C12Q1/04 C12Q1/06

    摘要: A method for semi-quantitatively or quantitatively detecting the presence of a microbe in a sample is provided. The method utilizes a test dye that undergoes a detectable color change in the presence of one or more microbes. For example, in one embodiment, the test dye is a solvatochromic dye (e.g., Reichardt's dye) that responds to differences in polarity between microbe components (e.g., cell membrane, cytoplasm, etc.) and the environment outside the cell. Alternatively, other mechanisms may be wholly or partially responsible for the interaction between the dye and the microbe, such as acid-base reactions, redox reactions, and so forth. Regardless, the color of the test dye may be compared to the color of a control dye, wherein the color of the control dye corresponds to a known microbe concentration.

    MICROBIAL DETECTION AND QUANTIFICATION
    8.
    发明公开
    MICROBIAL DETECTION AND QUANTIFICATION 有权
    细菌证明和量化

    公开(公告)号:EP1828402A2

    公开(公告)日:2007-09-05

    申请号:EP05851252.6

    申请日:2005-10-24

    IPC分类号: C12Q1/04 C12Q1/06

    摘要: A method for semi-quantitatively or quantitatively detecting the presence of a microbe in a sample is provided. The method utilizes a test dye that undergoes a detectable color change in the presence of one or more microbes. For example, in one embodiment, the test dye is a solvatochromic dye (e.g., Reichardt's dye) that responds to differences in polarity between microbe components (e.g., cell membrane, cytoplasm, etc.) and the environment outside the cell. Alternatively, other mechanisms may be wholly or partially responsible for the interaction between the dye and the microbe, such as acid-base reactions, redox reactions, and so forth. Regardless, the color of the test dye may be compared to the color of a control dye, wherein the color of the control dye corresponds to a known microbe concentration.

    MICROBIAL DETECTION AND QUANTIFICATION
    10.
    发明公开
    MICROBIAL DETECTION AND QUANTIFICATION 有权
    细菌证明和量化

    公开(公告)号:EP1694857A2

    公开(公告)日:2006-08-30

    申请号:EP04814617.9

    申请日:2004-12-16

    IPC分类号: C12Q1/04

    CPC分类号: C12Q1/04

    摘要: Suitable dyes are used herein to indicate the presence of microbial contamination by spraying them onto surfaces in the form of an aqueous solution. The dye solution may also be allowed to dry, thus producing the dried residue of an aqueous solution. It's believed that these dyes change color in response to a change in polarity of the environment. Since water is a polar solvent and most bacteria are made from non-polar substances, the presence of bacteria changes the polarity of the environment, triggering a change visible by the unaided eye.