公开(公告)号：EP1970448A1

公开(公告)日：2008-09-17

申请号：EP08010546.3

申请日：2002-05-10

申请人： Kirin Pharma Kabushiki Kaisha

发明人： Kuroiwa, Yoshimi ,  Tomizuka, Kazuma ,  Yoshida, Hitoshi ,  Ishida, Isao

IPC分类号： C12N15/09 ,  A01K67/027 ,  C07K16/00 ,  C12P21/08

CPC分类号： A01K67/0278 ,  A01K67/0275 ,  A01K67/0276 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/052 ,  A01K2217/054 ,  A01K2217/15 ,  A01K2227/105 ,  A01K2267/01 ,  A01K2267/0381 ,  A61K48/00 ,  A61K2039/505 ,  C07K16/00 ,  C07K16/243 ,  C07K2317/21 ,  C12N15/8509 ,  C12N15/87 ,  C12N15/90 ,  C12N2800/30

摘要： The present invention relates to a human artificial chromosome which is genetically transmissible to the next generation with high efficiency and the method for using the same. More specifically, the present invention relates to: a human artificial chromosome in which an about 3.5 Mb to about 1 Mb region containing an antibody λ light chain gene derived from human chromosome 22 is bound to a chromosome fragment which is transmissible to a progeny through a germ line of a non-human animal, said chromosome fragment is derived from another human chromosome; a non-human animal carrying the human artificial chromosome and an offspring thereof; a method for producing the non-human animal; a method for producing a human antibody using the non-human animal or an offspring thereof; and a human antibody-producing mouse carrying the human artificial chromosome.

摘要翻译： 本发明涉及一种人类人工染色体所有这些是具有高效率和使用该方法的基因传播给下一代。 更具体地，本发明涉及：其中A人类人工染色体至约3.5 MB至约1Mb的区域在抗体包含“来源于人22号染色体衍生的轻链基因被结合到染色体片段所有这些是透射通过到一个子代 非人类动物的种系，所述染色体片段从另一个人染色体衍生的; 非人类动物携带人类人工染色体和其后代; 用于制造非人类动物的方法; 使用非人动物或它们的后代上生产人抗体的方法; 和产生人抗体的小鼠携带人类人工染色体。

公开(公告)号：EP2042594A1

公开(公告)日：2009-04-01

申请号：EP08016978.2

申请日：2001-11-16

申请人： Kirin Pharma Kabushiki Kaisha

发明人： Robl, James M. ,  Kuroiwa, Yoshimi ,  Tomizuka, Kazuma ,  Ishida, Isao

IPC分类号： C12N15/00 ,  C12P21/00 ,  A01K67/033 ,  A01K67/027

CPC分类号： C12N15/8509 ,  A01K67/0273 ,  A01K67/0276 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2227/101 ,  A01K2267/01 ,  C12N9/1051 ,  C12N2800/30

摘要： The present invention relates to the production of a transgenic bovine which comprises a genetic modification that results in inactivation and loss of expression of its endogenous antibodies, and the expression of xenogenous antibodies, preferably human antibodies. This is effected by inactivation of the IgM heavy chain expression and, optionally, by inactivation of the Ig light chain expression, and by the further introduction of an artificial chromosome which results on the expression of non-bovine antibodies, preferably human antibodies.

摘要翻译： 本发明涉及转基因牛的生产，其包含导致其内源性抗体失活和丧失表达的遗传修饰，以及异源抗体（优选人抗体）的表达。 这通过IgM重链表达的失活和任选地通过Ig轻链表达的失活以及进一步引入导致非牛抗体（优选人抗体）表达的人造染色体来实现。