公开(公告)号：EP2274621A2

公开(公告)日：2011-01-19

申请号：EP09730039.6

申请日：2009-04-02

申请人： Koninklijke Philips Electronics N.V.

发明人： HOFFMANN, Ralf ,  ROMIJN, Edwin, P. ,  DIRKSEN, Eef, H. C.

IPC分类号： G01N33/68 ,  C07K1/13

CPC分类号： C07K1/13 ,  C12Q2563/167 ,  C40B20/04 ,  C40B70/00 ,  G01N33/58 ,  G01N33/6848 ,  G01N2458/15

摘要： The present invention relates to kits and methods for the isotopic/isobaric labeling and the subsequent affinity selection and analysis of proteinaceous molecules. In particular, the invention relates to a kit-of-parts comprising a combinatorial plurality of labeling reagent for the labeling of proteinaceous molecules, each labeling reagent comprising an isobaric label component, an isotopic label component, and a reactive group capable of reacting with a proteinaceous molecule, wherein the isotopic label component concomitantly is an affinity tag. The invention is also directed to a corresponding method for the analysis of proteinaceous molecules, comprising labeling at least one subset of the proteinaceous molecules present by employing a kit-of-parts as defined herein and subsequently separating the labeled molecules by affinity purification via the affinity tag comprised in the label. Finally, the invention relates to the uses of such methods for protein expression profiling or proteomic analyses.

公开(公告)号：EP2167973A2

公开(公告)日：2010-03-31

申请号：EP08789226.1

申请日：2008-07-08

申请人： Koninklijke Philips Electronics N.V.

发明人： HOFFMANN, Ralf ,  ROMIJN, Edwin, P. ,  DIRKSEN, Eef, H. C.

IPC分类号： G01N33/68

CPC分类号： G01N33/6848 ,  G01N2458/15 ,  H01J49/00

摘要： Subject of the present invention are new isotopic and isobaric tagged molecules, kits comprising them and methods of using them in mass spectrometry.

摘要翻译： 本发明的主题是新的同位素和同量异位标记分子，包含它们的试剂盒以及在质谱法中使用它们的方法。