公开(公告)号：EP2330186A2

公开(公告)日：2011-06-08

申请号：EP09814823.2

申请日：2009-09-22

Applicant: Korea Advanced Institute of Science and Technology

Inventor： LEE, Sang Yup ,  XIA, Xiaoxia ,  QIAN, Zhi Gang ,  BAEK, Jong Hwan

IPC: C12N1/21

CPC classification number: C12N15/67 ,  C07K14/43518 ,  C07K14/43586

Abstract: The present invention relates to a method of increasing the expression of a target protein by co-expression of a gene encoding a target protein having a high content of a specific amino acid with a nucleotide sequence encoding the tRNA of the specific amino acid. According to the present invention, the expression of a protein having a high content of a specific amino acid can be remarkably increased by co-expression with the tRNA of the specific amino acid. Thus, the present invention is useful for increasing the productivity of a protein having a high content of a specific amino acid, such as a repetitive protein.

Abstract translation: 本发明涉及由编码具有特定氨基酸的用编码特定氨基酸的tRNA的核苷酸序列的含量高的靶蛋白的基因的共表达增加靶蛋白的表达的方法。 。根据本发明，具有特定氨基酸的含量高的蛋白的表达可显着通过与特定的氨基酸的tRNA的联合表达增加。 因此，本发明是一种用于提高具有特定氨基酸的含量高，检查作为重复蛋白的蛋白质的生产率是有用的。

公开(公告)号：EP2330186B1

公开(公告)日：2014-12-31

申请号：EP09814823.2

申请日：2009-09-22

Applicant: Korea Advanced Institute of Science and Technology

Inventor： LEE, Sang Yup ,  XIA, Xiaoxia ,  QIAN, Zhi Gang ,  BAEK, Jong Hwan

IPC: C12N1/21 ,  C12N15/67 ,  C07K14/435

CPC classification number: C12N15/67 ,  C07K14/43518 ,  C07K14/43586