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    METHOD FOR INDUCING DIFFERENTIATION OF ALVEOLAR EPITHELIAL CELLS
    2.
    发明公开
    METHOD FOR INDUCING DIFFERENTIATION OF ALVEOLAR EPITHELIAL CELLS 审中-公开
    诱导肺泡上皮细胞分化的方法

    公开(公告)号：EP3266864A1

    公开(公告)日：2018-01-10

    申请号：EP16761771.1

    申请日：2016-03-02

    申请人： Kyoto University

    发明人： GOTOH, Shimpei ,  YAMAMOTO, Yuki ,  KONISHI, Satoshi ,  MISHIMA, Michiaki

    IPC分类号： C12N5/074 ,  C12N5/071 ,  C12N5/073 ,  C12N5/0735 ,  C12N5/076

    CPC分类号： C12N5/0688 ,  C12N2501/117 ,  C12N2501/119 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/39 ,  C12N2501/415 ,  C12N2501/50 ,  C12N2501/727 ,  C12N2501/73 ,  C12N2506/45 ,  C12N2513/00

    摘要： This invention provides a method for stably producing type II alveolar epithelial cells from pluripotent stem cells. Specifically, the invention relates to a method for producing type II alveolar epithelial cells from pluripotent stem cells comprising steps of: (1) culturing pluripotent stem cells in a medium containing activin A and a GSK3β inhibitor; (2) culturing the cells obtained in Step (1) in a medium containing a BMP inhibitor and a TGFβ inhibitor; (3) culturing the cells obtained in Step (2) in a medium containing BMP4, retinoic acid, and a GSK3β inhibitor; (4) culturing the ventral anterior foregut cells obtained in Step (3) in a medium containing a GSK3β inhibitor, FGF10, KGF, and a NOTCH signal inhibitor; and (5) subjecting the alveolar epithelial progenitor cells obtained in Step (4) to three-dimensional culture in a medium containing a steroid drug, a cAMP derivative, a phosphodiesterase inhibitor, and KGF.

    摘要翻译： 本发明提供了一种从多能干细胞稳定生产II型肺泡上皮细胞的方法。 具体而言，本发明涉及由多能干细胞制备II型肺泡上皮细胞的方法，其包括以下步骤：（1）在含有激活素A和GSK3β抑制剂的培养基中培养多能干细胞; （2）在含有BMP抑制剂和TGFβ抑制剂的培养基中培养步骤（1）中获得的细胞; （3）在含有BMP4，视黄酸和GSK3β抑制剂的培养基中培养步骤（2）中获得的细胞; （4）在含有GSK3β抑制剂，FGF10，KGF和NOTCH信号抑制剂的培养基中培养步骤（3）中得到的腹侧前肠细胞; （5）使步骤（4）中获得的肺泡上皮祖细胞在含有类固醇药物，cAMP衍生物，磷酸二酯酶抑制剂和KGF的培养基中进行三维培养。

    METHOD FOR INDUCING DIFFERENTIATION OF AIRWAY EPITHELIAL CELLS
    4.
    发明公开
    METHOD FOR INDUCING DIFFERENTIATION OF AIRWAY EPITHELIAL CELLS 审中-公开
    诱导气道上皮细胞分化的方法

    公开(公告)号：EP3272859A1

    公开(公告)日：2018-01-24

    申请号：EP16765134.8

    申请日：2016-03-18

    申请人： Kyoto University

    发明人： GOTOH, Shimpei ,  YAMAMOTO, Yuki ,  KONISHI, Satoshi ,  MISHIMA, Michiaki

    IPC分类号： C12N5/22 ,  C12N5/0735

    摘要： This invention provides a method for stably producing airway epithelial cells from pluripotent stem cells. Specifically, the invention relates to a method for producing airway epithelial cells from pluripotent stem cells comprising steps: (1) culturing pluripotent stem cells in a medium containing activin A and a GSK3β inhibitor; (2) culturing the cells obtained in Step (1) in a medium containing a BMP inhibitor and a TGFβ inhibitor; (3) culturing the cells obtained in Step (2) in a medium containing BMP4, retinoic acid, and a GSK3β inhibitor; (5) subjecting the cells obtained after Step (3) to three-dimensional culture in a medium containing a GSK3β inhibitor, FGF10, and a ROCK inhibitor; and (6) subjecting the proximal airway epithelial progenitor cells obtained in Step (5) to three-dimensional culture in a medium containing a ROCK inhibitor.

    摘要翻译： 本发明提供了一种从多能干细胞稳定产生气道上皮细胞的方法。 具体而言，本发明涉及由多能干细胞制造气道上皮细胞的方法，其包括以下步骤：（1）在含有激活素A和GSK3β抑制剂的培养基中培养多能干细胞; （2）在含有BMP抑制剂和TGFβ抑制剂的培养基中培养步骤（1）中获得的细胞; （3）在含有BMP4，视黄酸和GSK3β抑制剂的培养基中培养步骤（2）中获得的细胞; （5）将步骤（3）后获得的细胞在含有GSK3β抑制剂，FGF10和ROCK抑制剂的培养基中进行三维培养; （6）使步骤（5）中获得的近端气道上皮祖细胞在含有ROCK抑制剂的培养基中进行三维培养。