公开(公告)号：EP2865762A1

公开(公告)日：2015-04-29

申请号：EP13807729.2

申请日：2013-06-14

申请人： Kyushu University, National University Corporation ,  Hitachi Aloka Medical, Ltd.

发明人： KAMIYA, Noriho ,  HAYASHI, Kounosuke ,  NAGAI, Kenji

IPC分类号： C12P19/34 ,  C12Q1/68 ,  G01N33/53

CPC分类号： C12Q1/6804 ,  C12P19/34 ,  C12P21/02 ,  G01N33/5308 ,  G01N33/532 ,  G01N33/58 ,  G01N33/582 ,  C12Q2521/119 ,  C12Q2521/131 ,  C12Q2525/205 ,  C12Q2563/119

摘要： Provided is a method for producing a nucleic acid probe that can detect a target substance with good sensitivity. A method for producing a nucleic acid probe, comprising: a 3'-terminal addition step of adding at least one nucleoside triphosphate derivative having a glutamine (Gin) residue or a lysine (Lys) residue to the 3'-terminal of a nucleic acid using a 3'-terminal addition enzyme that can add a nucleotide to the 3'-terminal of a nucleic acid; and a labeling compound binding step of binding a labeling compound having a lysine (Lys) residue and containing a labeling moiety to the glutamine (Gin) residue using a transglutaminase (TGase) or binding a labeling compound having a glutamine (Gin) residue and containing a labeling moiety to the lysine (Lys) residue using a transglutaminase (TGase).

摘要翻译： 提供了一种用于制造核酸探针也可以检测具有良好的灵敏度的目标物质的方法。 一种用于生产核酸样品的方法，包括：加入至少一种核苷三磷酸衍生物具有谷氨酰胺（Gln）残基或赖氨酸（Lys）残基的核酸的3“末端的3'末端添加步骤 使用3'末端加入酶可以做一个核苷酸添加到核酸的3'-末端; 和标记化合物结合的结合的标记化合物具有赖氨酸（Lys）残基和含有标记部分的谷氨酰胺使用转谷氨酰胺酶（TGase）（Gln）残基或结合具有谷氨酰胺（Gln）残基的标记化合物和含有步骤 标记部分使用转谷氨酰胺酶（TGase）的赖氨酸（Lys）残基。

公开(公告)号：EP3059250A1

公开(公告)日：2016-08-24

申请号：EP14854395.2

申请日：2014-10-06

申请人： Kyushu University, National University Corporation ,  Hitachi, Ltd.

发明人： KAMIYA, Noriho ,  MATSUBA, Kyoichi ,  NAGAI, Kenji ,  HAYASHI, Kounosuke

IPC分类号： C07K19/00 ,  C12Q1/42 ,  G01N33/68 ,  C07K14/315 ,  C12N9/16 ,  C12N15/09

CPC分类号： C12N9/16 ,  C07K2319/00 ,  C07K2319/21 ,  C07K2319/61 ,  C12Y301/03001 ,  G01N33/6803

摘要： A fusion protein for protein detection in which are fused: a protein domain including at least one among a C1 domain of protein G, a C2 domain of protein G, and a C3 domain of protein G; and a double mutant D153G/D330N of Escherichia coli alkaline phosphatase (BAP) in which the 153 amino acid residue Asp has been substituted by Gly and the 330 amino acid residue Asp has been substituted by Asn, a double mutant D153H/D330N of Escherichia coli alkaline phosphatase (BAP) in which the 153 amino acid residue Asp has been substituted by His and the 330 amino acid residue Asp has been substituted by Asn, or a double mutant K328R/D330N of Escherichia coli alkaline phosphatase (BAP) in which the 328 amino acid residue Lys has been substituted by Arg and the 330 amino acid residue Asp has been substituted by Asn.