公开(公告)号：EP3268732A1

公开(公告)日：2018-01-17

申请号：EP16762680.3

申请日：2016-03-11

申请人： Two Pore Guys, Inc.

发明人： MORIN, Trevor, J.

IPC分类号： G01N33/00 ,  G01N33/48 ,  G01N33/50 ,  G01N33/53

CPC分类号： G01N33/48721 ,  C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6869 ,  G01N33/54306 ,  G01N33/54366 ,  C12Q2563/119 ,  C12Q2563/155 ,  C12Q2563/167 ,  C12Q2565/631

摘要： Disclosed herein are methods and compositions for detection of target small molecules in a mixed sample by performing a competition assay between the target and a surrogate and subsequently detecting the complex types in a nanopore device.

公开(公告)号：EP2765424A1

公开(公告)日：2014-08-13

申请号：EP12838806.3

申请日：2012-10-04

申请人： Hitachi High-Technologies Corporation

发明人： SAITO, Toshiro ,  HAMASAKI, Koshin ,  TAKAHASHI, Satoshi ,  MAESHIMA, Muneo ,  IMAI, Kyoko ,  IMAI, Kazumichi ,  TAO, Ryuji

IPC分类号： G01N33/553 ,  C12M1/00 ,  C12Q1/68 ,  G01N21/64 ,  G01N33/543

CPC分类号： C12Q1/6834 ,  C12Q1/6804 ,  C12Q1/6837 ,  G01N21/6428 ,  G01N21/6452 ,  G01N33/54326 ,  G01N33/54346 ,  G01N33/54393 ,  G01N33/587 ,  G01N2021/6421 ,  C12Q2525/161 ,  C12Q2545/114 ,  C12Q2563/107 ,  C12Q2563/143 ,  C12Q2563/149 ,  C12Q2565/514 ,  C12Q2563/119

摘要： An object of the present invention is to provide a method and means for analyzing biomolecules that can realize, in biomolecule analysis, a wide dynamic range attained by counting the number of biomolecules and rapid analysis. The present invention relates to a method for analyzing biomolecules, comprising the steps of: immobilizing biomolecules 101 to be analyzed on surfaces of magnetic microparticles 108; reacting labeled probe molecules 104 with the biomolecules 101 to be analyzed; collecting and immobilizing the microparticles 108 on a support substrate 110; and measuring a label on the support substrate 110. Since single-molecule immobilized magnetic microparticles are used in the present invention, the number of biomolecules can be counted, and since hybridization and an antigen-antibody reaction are performed with the microparticles having biomolecules immobilized thereon dispersed, the reaction can be rapidly performed. Further, the type and the abundance of biomolecules of interest can be determined at a single molecular level, so as to evaluate, in particular, the absolute concentration of biomolecules.

摘要翻译： 本发明的目的是提供一种用于分析生物分子的方法和装置，其可以在生物分子分析中实现通过计数生物分子数量和快速分析获得的宽动态范围。 本发明涉及一种分析生物分子的方法，其特征在于包括以下步骤：将待分析的生物分子101固定在磁性微粒108的表面上; 使标记的探针分子104与待分析的生物分子101反应; 将微粒108收集并固定在支撑基板110上; 并测量支撑基板110上的标签。由于在本发明中使用单分子固定的磁性微粒，因此可以计数生物分子的数量，并且由于在其上固定有生物分子的微粒进行杂交和抗原 - 抗体反应 分散，可迅速进行反应。 此外，感兴趣的生物分子的类型和丰度可以在单个分子水平上确定，以便特别地评估生物分子的绝对浓度。

公开(公告)号：EP1836319B1

公开(公告)日：2012-09-19

申请号：EP06717401.1

申请日：2006-01-06

申请人： Life Technologies Corporation

发明人： MARTIN, Patrick K. ,  SIMPSON, David A. ,  HARDY, Christine D.

IPC分类号： C12Q1/68 ,  C07K14/195 ,  C12N15/62 ,  C12N9/12

CPC分类号： C12P19/34 ,  C07K14/195 ,  C07K2319/71 ,  C07K2319/80 ,  C12N9/1252 ,  C12N9/96 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/6846 ,  C12Q2522/101 ,  C12Q2521/101 ,  C12Q2563/119 ,  C12Q2527/101

公开(公告)号：EP2301946A1

公开(公告)日：2011-03-30

申请号：EP09800477.3

申请日：2009-07-22

申请人： Kyushu University, National University Corporation ,  The University of Tokushima ,  Aloka Co., Ltd.

发明人： KAMIYA, Noriho ,  NOJI, Sumihare ,  HIRAISHI, Yoshiyuki

IPC分类号： C07H19/067 ,  C12N15/09 ,  C12Q1/68

CPC分类号： C07H19/067 ,  C07H19/10 ,  C07H19/207 ,  C12Q1/6816 ,  C12Q2563/119 ,  C12Q2563/125

摘要： A novel nucleoside triphosphate derivative, a nucleic acid probe, and a multilabeled nucleic acid probe that can detect a target nucleic acid conveniently and with high sensitivity, as well as a method for producing the multilabeled nucleic acid probe, and a method for detecting a target nucleic acid using the multilabeled nucleic acid probe or the nucleic acid probe. A target nucleic acid can be detected conveniently and with high sensitivity by using a transglutaminase (TGase), and by using a multilabeled nucleic acid probe in which a plurality of labeling portions have been introduced in advance by covalent binding, or by introducing a plurality of labeling portions by covalent binding into a nucleic acid probe that has been hybridized with the target nucleic acid.

摘要翻译： 一种新型核苷三磷酸衍生物，核酸探针，和一个多标记核酸探针做了能够简便且高灵敏度，以及一种制备该多标记核酸探针的方法，和用于检测靶的方法检测靶核酸 核酸使用多标记核酸探针或该核酸样品。 靶核酸可以方便，高灵敏度，通过使用转谷氨酰胺酶（TGase），以及通过使用其中标记部分的多元性havebeen预先共价结合引入了一个多标记核酸探针来检测，或通过引入的多元性 共价结合到核酸探针标记部分也已杂交的靶核酸。

公开(公告)号：EP1833969B1

公开(公告)日：2010-12-08

申请号：EP06717485.4

申请日：2006-01-06

申请人： Life Technologies Corporation

发明人： MARTIN, Patrick, K. ,  SIMPSON, David, A.

IPC分类号： C12N15/62

CPC分类号： C07K14/00 ,  C07K14/195 ,  C07K2319/80 ,  C12N9/1252 ,  C12Q1/6832 ,  C12Y207/07007 ,  C12Q2563/119

摘要： Polypeptides having nucleic acid binding activity are provided. Methods of stabilizing a nucleic acid duplex are provided. Methods of promoting the annealing of complementary nucleic acid strands are provided. Methods of increasing the processivity of a DNA polymerase are provided. Methods of enhancing the activity of a nucleic acid modification enzyme are provided. Fusion proteins are provided. Methods of using fusion proteins are provided. Kits are provided.

摘要翻译： 提供了具有核酸结合活性的多肽。 提供了稳定核酸双链体的方法。 提供了促进互补核酸链退火的方法。 提供增加DNA聚合酶的持续性的方法。 提供增强核酸修饰酶活性的方法。 提供融合蛋白。 提供使用融合蛋白的方法。 提供套件。

公开(公告)号：EP1394270A3

公开(公告)日：2004-05-06

申请号：EP03018640.7

申请日：2003-08-20

申请人： Bioarray Solutions Ltd

发明人： Yang, Jiacheng

IPC分类号： C12Q1/68 ,  G01N33/543 ,  C12Q1/25

CPC分类号： C12Q1/6837 ,  C07K14/003 ,  C07K2319/50 ,  C12N2310/3181 ,  C12Q1/37 ,  C12Q1/42 ,  C12Q1/48 ,  C12Q1/485 ,  C12Q1/6827 ,  G01N33/54366 ,  G01N33/6893 ,  G01N33/6896 ,  C12Q2525/107 ,  C12Q2525/161 ,  C12Q2537/137 ,  C12Q2565/518 ,  C12Q2563/119

摘要： The present invention relates to molecular constructs and methods of their use in detecting biochemical reactions. In particular, the invention relates to a molecular construct having a capture portion and a substrate portion, where the capture portion isolates the construct from a sample medium, and the substrate portion enables the construct to be acted upon and undergo a physical change which can be detected and measured. These molecular constructs may be used in diagnostic assays, genetic screening for potential risks of certain diseases in individuals, and drug discovery and toxicogenomics, using high throughput screening of compounds.

公开(公告)号：EP1144692A1

公开(公告)日：2001-10-17

申请号：EP99973074.0

申请日：1999-11-30

申请人： Nycomed Pharma A/S ,  Grant, Anne Rosemary

发明人： IHLE, Oystein ,  MICHAELSEN, Terje, Einar ,  JOHNE, Berit

IPC分类号： C12Q1/68 ,  C12N15/10

CPC分类号： C12N15/1006 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q2563/131 ,  C12Q2525/131 ,  C12Q2522/101 ,  C12Q2563/119

摘要： A method for at least partially separating nucleic acid molecules in a sample into populations wherein a population is tagged or capable of being tagged with a moiety capable of being immobilised on a matrix, said method comprising contacting the nucleic acid containing sample with a matrix whereby the tagged molecules are captured by the matrix and thereby separated from untagged molecules. The method may be used in a variety of applications, including separating restriction enzyme digestion products, separating linear from circular nucleic acid molecules, in vitro packaging of bacteriophage particles, diagnostic PCR.

公开(公告)号：EP0834576A2

公开(公告)日：1998-04-08

申请号：EP97116548.5

申请日：1991-12-06

申请人： AFFYMAX TECHNOLOGIES N.V.

发明人： Fodor, Stephen P.A. ,  Dower, William J. ,  Solas, Dennis W.

IPC分类号： C12Q1/68 ,  C07H21/00

CPC分类号： G03F7/00 ,  B01J19/0046 ,  B01J2219/00315 ,  B01J2219/00432 ,  B01J2219/00434 ,  B01J2219/00436 ,  B01J2219/00459 ,  B01J2219/00468 ,  B01J2219/00475 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00531 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00725 ,  B82Y10/00 ,  B82Y30/00 ,  C07B2200/11 ,  C07H19/10 ,  C07H21/00 ,  C07K1/042 ,  C07K1/045 ,  C07K17/06 ,  C07K17/14 ,  C12Q1/6804 ,  C12Q1/6809 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q1/6874 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N15/1475 ,  G03F7/265 ,  G03F7/38 ,  G11C13/0014 ,  G11C13/0019 ,  C12Q2565/513 ,  C12Q2565/507 ,  C12Q2545/101 ,  C12Q2525/185 ,  C12Q2565/514 ,  C12Q2563/173 ,  C12Q2563/119 ,  C12Q2563/149 ,  C12Q2525/151 ,  C12Q2525/179 ,  C12Q2525/204

摘要： The present invention provides methods and apparatus for sequencing, fingerprinting and mapping biological polymers, particularly polynucleotides. The methods make use of a plurality of positionally distinct sequence specific recognition reagents, such as polynucleotides. The apparatus employs a substrate comprising positionally distinct sequence specific recognition reagents, such as polynucleotides, which are preferably localized at high densities. The methods and apparatus of the present invention can be used for determining the sequence of polynucleotides, mapping polynucleotides, and developing polynucleotide fingerprints. Polynucleotide fingerprints can be used for identifying individuals, tissue samples, pathological conditions, genetic diseases, infectious diseases, and other applications. Polynucleotide fingerprints can also be used for classification of biological samples, including taxonomy, and to characterize their sources. The invention also provides polynucleotide mapping, fingerprinting, and sequencing as valuable laboratory research tools for use in biological investigations.

公开(公告)号：EP0834575A2

公开(公告)日：1998-04-08

申请号：EP97116541.0

申请日：1991-12-06

申请人： AFFYMAX TECHNOLOGIES N.V.

发明人： Fodor, Stephen P. A. ,  Dower, William J. ,  Solas, Dennis W.

IPC分类号： C12Q1/68 ,  C07H21/00

CPC分类号： G03F7/00 ,  B01J19/0046 ,  B01J2219/00315 ,  B01J2219/00432 ,  B01J2219/00434 ,  B01J2219/00436 ,  B01J2219/00459 ,  B01J2219/00468 ,  B01J2219/00475 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00531 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00725 ,  B82Y10/00 ,  B82Y30/00 ,  C07B2200/11 ,  C07H19/10 ,  C07H21/00 ,  C07K1/042 ,  C07K1/045 ,  C07K17/06 ,  C07K17/14 ,  C12Q1/6804 ,  C12Q1/6809 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q1/6874 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N15/1475 ,  G03F7/265 ,  G03F7/38 ,  G11C13/0014 ,  G11C13/0019 ,  C12Q2565/513 ,  C12Q2565/507 ,  C12Q2545/101 ,  C12Q2525/185 ,  C12Q2565/514 ,  C12Q2563/173 ,  C12Q2563/119 ,  C12Q2563/149 ,  C12Q2525/151 ,  C12Q2525/179 ,  C12Q2525/204

摘要： The present invention provides methods and apparatus for sequencing, fingerprinting and mapping biological polymers, particularly polynucleotides. The methods make use of a plurality of positionally distinct sequence specific recognition reagents, such as polynucleotides. The apparatus employs a substrate comprising positionally distinct sequence specific recognition reagents, such as polynucleotides, which are preferably localized at high densities. The methods and apparatus of the present invention can be used for determining the sequence of polynucleotides, mapping polynucleotides, and developing polynucleotide fingerprints. Polynucleotide fingerprints can be used for identifying individuals, tissue samples, pathological conditions, genetic diseases, infectious diseases, and other applications. Polynucleotide fingerprints can also be used for classification of biological samples, including taxonomy, and to characterize their sources. The invention also provides polynucleotide mapping, fingerprinting, and sequencing as valuable laboratory research tools for use in biological investigations.

摘要翻译： 本发明提供了用于测序，指纹和绘制生物聚合物，特别是多核苷酸的方法和装置。 所述方法利用多种位置不同的序列特异性识别试剂，例如多核苷酸。 该装置使用包含位置不同的序列特异性识别试剂（例如多核苷酸）的底物，其优选以高密度定位。 本发明的方法和装置可以用于确定多核苷酸序列，映射多核苷酸和开发多核苷酸指纹。 多核苷酸指纹可用于鉴定个体，组织样本，病理状况，遗传疾病，传染病和其他应用。 多核苷酸指纹也可用于生物样品的分类，包括分类学，以及其来源特征。 本发明还提供多核苷酸测绘，指纹图谱和测序作为用于生物学研究的宝贵的实验室研究工具。

公开(公告)号：EP0453301A3

公开(公告)日：1993-07-21

申请号：EP91303516.8

申请日：1991-04-19

申请人： BEHRINGWERKE Aktiengesellschaft

发明人： Ullman, Edwin F. ,  Goodman, Thomas C.

IPC分类号： C12Q1/68 ,  G01N33/50

CPC分类号： C12Q1/6804 ,  C12Q1/6813 ,  C12Q1/6816 ,  Y10S435/81 ,  Y10S530/81 ,  C12Q2537/125 ,  C12Q2522/101 ,  C12Q2563/119 ,  C12Q2521/501

摘要： A method for the detection of a polynucleotide target sequence is described. The method involves the formation of a covalent or non-covalent bonded pair of nucleotide sequences formed in response to a target polynucleotide sequence, adding nucleotide sequence specific binding proteins each capable of binding one member of the pair of nucleotide sequences, and detecting the specific binding proteins complexed to the pair of nucleotide sequences.