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1.发明公开CAP-BINDING DOMAIN OF HUMAN EUKARYOTIC PROTEIN SYNTHESIS INITIATION FACTOR eIF-4E AND THE USE THEREOF 失效CAP-结合区的人真核蛋白的合成起始因子EIF-4E及其应用
公开(公告)号：EP0975652A1
公开(公告)日：2000-02-02
申请号：EP97942384.5
申请日：1997-09-02
发明人： GOSS, Dixie, J. , FRIEDLAND, Diana
CPC分类号： C07K14/4705
摘要： Binding of eIF-4E to the 5'm7G cap structure of eukaryotic mRNA signals the initiation of protein synthesis. In the present invention, the binding site cap-binding domain of eIF-4E was identified as the region containing the sequence Trp?113 to Arg122¿. Thus, in a first preferred embodiment the present invention provides a truncated peptide of the cap binding protein consisting essentially of the mRNA cap-binding domain. Specifically, the invention concerns eIF-4E cap binding peptide and its variants. In additional preferred embodiments, the invention provides an isolated nucleic acid molecule which encodes a truncated peptide of the eIF-4E protein consisting essentially of the mRNA cap-binding domain of eIF-4E; a recombinant vector and host cell comprising the above-described nucleic acid molecule; and a recombinant peptide produced thereby. The invention further provides the truncated or recombinant peptides immobilized on a solid support, a resin comprising the truncated or recombinant peptides immobilized on a solid support, methods and kits for isolating a capped mRNA molecule, and methods and kits for producing a full-length cDNA molecule.
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