公开(公告)号：EP2403843A4

公开(公告)日：2012-08-15

申请号：EP10749157

申请日：2010-03-01

申请人： LIFE TECHNOLOGIES CORP

发明人： JUO ROUH-RONG ,  BROOKS EDWARD ,  GEE MELISSA ,  WANG ZHIXIAN H ,  SKAARE KATHLEEN

IPC分类号： C07D321/00 ,  C07D407/04

CPC分类号： C12Q1/26 ,  C07D305/14 ,  C07D321/00 ,  C07D323/00 ,  C07D407/04 ,  C07D409/04 ,  C07D493/04 ,  C07D493/10 ,  G01N2333/90245 ,  G01N2333/90251 ,  G01N2333/90258

摘要： Chemiluminescent compositions, methods, assays and kits for oxidative enzymes are described. Further disclosed are dioxetane compounds of the form: where R can independently be any branched alkyl or cycloalkyl group which provides stabilization for the dioxetane or where both R groups together form a cycloalkyl or polycycloalkyl moiety spiro bound to the dioxetane ring, wherein each R group or the spiro bound moiety can be unsubstituted or substituted with one or more electron-withdrawing groups or electron donating groups, or groups providing preferential oxidative isozyme substrate recognition, and wherein R1 is an aryl group, or an alkyl group of 1-20 carbon atoms, which can be optionally substituted with 1 or more halogen atoms, and wherein T is an aryl or heteroaryl ring capable of emitting light upon enzyme activated decomposition of the dioxetane I. Kits, methods and assays are also disclosed that comprise the dioxetane compounds.

摘要翻译： 还公开了包含二恶丁环化合物的试剂盒，方法和测定法。

公开(公告)号：EP2590958A4

公开(公告)日：2014-01-01

申请号：EP11804293

申请日：2011-07-06

申请人： LIFE TECHNOLOGIES CORP

发明人： SPARKS ALISON ,  WANG ZHIXIAN ,  GEE MELISSA

IPC分类号： C07D305/00 ,  C07D305/14 ,  C07F9/00

CPC分类号： G01N33/581 ,  C07D321/00 ,  C07F9/12 ,  C07F9/4056 ,  C07F9/6541 ,  C07F9/65512 ,  C07F9/65583 ,  C07H15/203 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/6816

摘要： Methods for generating a chemiluminescent enzyme substrate in situ, in aqueous or other assay conditions. Also disclosed are methods to use the substrates to generate light, detect and/or quantify enzymes, antigens, and/or nucleic acids. Kits relating to these methods are also disclosed.