公开(公告)号：EP0350962A2

公开(公告)日：1990-01-17

申请号：EP89112988.4

申请日：1989-07-14

申请人： LIFE TECHNOLOGIES INC.

发明人： Flesher, Robert W. ,  Berninger, Mark S. ,  Blakesley, Robert W.

IPC分类号： G01N27/26

CPC分类号： G01N27/44704

摘要： An apparatus provides for casting a gel and for presenting the gel for electrophoresis. The apparatus comprises a casting tray and the casting plate which combine to form a mold for the gelable liquid, and an electrophoresis tank in which the electrophoresis process occurs. The floor of the electro­phoresis tank rises to form the plate pedestal upon which the casting plate rests during electrophoresis. The floor of the electrophoresis tank forms a cavity at the edges of the plate pedestal in which the casting tray rests during the electro­phoresis process. The casting plate is first firmly seated in the bottom of the casting tray forming a mold for the gelable liquid. The gelable liquid is poured into the mold. The comb is placed in the comb slots so as to form wells in the gel. The gelable liquid is allowed to gel, and the comb is removed. The material to undergo electrophoresis is placed in the wells left by the comb. The entire assembly is then placed and aligned on the plate pedestal. The casting tray is then pushed down into the casting tray well. Electrophoresis buffer sufficient to fill the tank to approximately the top of the gel is then poured into the electrophoresis tank. An alternate embodiment is shown.

摘要翻译： 一种装置提供了凝胶凝胶并呈现凝胶进行电泳。 该装置包括铸造托盘和组合形成用于可凝胶化液体的模具的流延板和其中发生电泳过程的电泳槽。 电泳槽的地板上升，形成电镀板上放置板的底座。 电泳槽的底部在板基座的边缘形成一个空腔，其中铸造托盘在电泳过程中搁置。 铸造板首先牢固地安置在铸造托盘的底部，形成用于可凝胶化液体的模具。 将可凝胶的液体倒入模具中。 将梳子放置在梳子槽中，以便在凝胶中形成孔。 使凝胶状液体凝胶化并除去梳子。 将进行电泳的材料置于由梳子留下的孔中。 然后将整个组件放置并对准在板基座上。 然后将铸造托盘向下推入铸造盘中。 然后将足以将罐填充至凝胶顶部的电泳缓冲液倒入电泳槽中。 示出了替代实施例。

公开(公告)号：EP0350962A3

公开(公告)日：1990-10-17

申请号：EP89112988.4

申请日：1989-07-14

申请人： LIFE TECHNOLOGIES INC.

发明人： Flesher, Robert W. ,  Berninger, Mark S. ,  Blakesley, Robert W.

IPC分类号： G01N27/26

CPC分类号： G01N27/44704

摘要： An apparatus provides for casting a gel and for presenting the gel for electrophoresis. The apparatus comprises a casting tray and the casting plate which combine to form a mold for the gelable liquid, and an electrophoresis tank in which the electrophoresis process occurs. The floor of the electro­phoresis tank rises to form the plate pedestal upon which the casting plate rests during electrophoresis. The floor of the electrophoresis tank forms a cavity at the edges of the plate pedestal in which the casting tray rests during the electro­phoresis process. The casting plate is first firmly seated in the bottom of the casting tray forming a mold for the gelable liquid. The gelable liquid is poured into the mold. The comb is placed in the comb slots so as to form wells in the gel. The gelable liquid is allowed to gel, and the comb is removed. The material to undergo electrophoresis is placed in the wells left by the comb. The entire assembly is then placed and aligned on the plate pedestal. The casting tray is then pushed down into the casting tray well. Electrophoresis buffer sufficient to fill the tank to approximately the top of the gel is then poured into the electrophoresis tank. An alternate embodiment is shown.

摘要翻译： 一种装置用于铸造凝胶并呈现用于电泳的凝胶。 该设备包括结合形成用于可胶凝液体的模具的浇铸盘和浇铸板以及发生电泳过程的电泳槽。 电泳槽的底板上升以形成电泳期间浇铸板搁置的板基座。 电泳槽的底板在电泳过程期间在其中放置托盘的平板基座的边缘处形成空腔。 首先将浇铸板牢固地安置在浇铸托盘的底部，形成用于可胶凝液体的模具。 将可胶凝液体倒入模具中。 将梳子放置在梳齿槽中以便在凝胶中形成孔。 使可胶凝液体凝胶化，并将梳子取出。 将进行电泳的材料放置在梳子留下的孔中。 然后将整个组件放置并对齐在板基座上。 然后将铸造托盘向下推入铸造托盘。 然后将电泳缓冲液倒入电泳槽中，所述电泳缓冲液足以将容器充满到大约凝胶的顶部。 显示了一个替代实施例。