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    COMPOSITIONS AND METHODS FOR ENHANCED SYNTHESIS OF NUCLEIC ACID MOLECULES
    9.
    发明公开
    COMPOSITIONS AND METHODS FOR ENHANCED SYNTHESIS OF NUCLEIC ACID MOLECULES 有权
    化合物和方法VERTÄARKERRN核酸分子合酶

    公开(公告)号：EP1062360A1

    公开(公告)日：2000-12-27

    申请号：EP99914904.0

    申请日：1999-03-15

    申请人： Life Technologies, Inc.

    发明人： LI, Wu-Bo ,  JESSEE, Joel, A. ,  SCHUSTER, David ,  XIA, Jiulin ,  GEBEYEHU, Gulilat

    IPC分类号： C12P19/34 ,  C07H21/04

    CPC分类号： C12P19/34 ,  C07H21/00 ,  C12Q1/6848 ,  C12Q2527/125

    摘要： The present invention is directed to compositions and methods for enhancing synthesis of nucleic acid molecules, particularly GC-rich nucleic acid molecules. Specifically, the invention provides compositions comprising one or more nitrogen-containing organic compounds having a formula selected from the group consisting of formula I and formula II (or salts or derivatives thereof), preferably 4-methylmorpholine N-oxide or betaine (carboxymethyltrimethylammonium), and further comprising one or more compounds selected from the group consisting of proline and an N-alkylimidazole compound, and more preferably proline, 1-methylimidazole or 4-mehtylimidazole. The invention further relates to methods for enhanced, high-fidelity synthesis of nucleic acid molecules, including via amplification (particularly PCR), reverse transcription, and sequencing methods. The invention also relates to nucleic acid molecules synthesized by these methods, to fragments or derivatives thereof, and to vectors and host cells comprising such nucleic acid molecules, fragments, or derivatives. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the compositions of the invention.

    METHODS FOR PRODUCTION AND PURIFICATION OF NUCLEIC ACID MOLECULES
    10.
    发明公开
    METHODS FOR PRODUCTION AND PURIFICATION OF NUCLEIC ACID MOLECULES 失效
    方法用于生产和核酸分子的清洁

    公开(公告)号：EP0981535A4

    公开(公告)日：2000-11-29

    申请号：EP98922202

    申请日：1998-05-12

    申请人： LIFE TECHNOLOGIES INC

    发明人： GRUBER CHRISTIAN E ,  JESSEE JOEL A

    IPC分类号： C12N15/09 ,  C07H21/00 ,  C07H21/04 ,  C12N15/10 ,  C12P19/34 ,  C12Q1/68

    CPC分类号： C12N15/101 ,  C12N15/1003 ,  C12N15/1096

    摘要： The present invention is directed to methods for the production and isolation of nucleic acid molecules. In particular, the invention concerns isolation of mRNA molecules and the production and isolation of nucleic acid molecules (e.g., cDNA molecules or libraries), which may be single- or double-stranded. Additionally, the invention concerns selection and isolation of particular nucleic acid molecules of interest from a sample which may contain a population of molecules. Specifically, the invention concerns affinity-labeled primer-adapter molecules which allow improved isolation and production of such nucleic acid molecules, increasing both product recovery and speed of isolation.