公开(公告)号：EP0318143A3

公开(公告)日：1989-06-07

申请号：EP88309438.5

申请日：1988-10-10

申请人： LUBRIZOL GENETICS INC.

发明人： Sekar, Vaithlingham Department of Biotechnology ,  Adang, Michael J.

IPC分类号： C12N15/00 ,  C12P21/02 ,  C12N1/20

CPC分类号： C07K14/325

摘要： The present invention relates to a novel biocontrol protein which is a crystal toxin and an insecticide and which is produced in a strain of bacteria genetically altered upon incorporation of a specific gene inserted as a stable gene construction. In particular, the present invention relates to the insertion of the δ-endotoxin gene from B. thuringiensis var. tenebrionis into a cloning vector containing prokaryotic regulatory and replication systems and the utilization of this vector to transform bacterial strains into coleopteran pathogens. Limited deletion of the coding region of the gene of this invention still allows preservation of the insecticidal activity in the protein expressed. In addition, this invention provides genetically altered bacterial strains that are insect toxic and that can be used as a novel method of biological control of agricultural insect pests.

公开(公告)号：EP0318143A2

公开(公告)日：1989-05-31

申请号：EP88309438.5

申请日：1988-10-10

申请人： LUBRIZOL GENETICS INC.

发明人： Sekar, Vaithlingham Department of Biotechnology ,  Adang, Michael J.

IPC分类号： C12N15/00 ,  C12P21/02 ,  C12N1/20

CPC分类号： C07K14/325

摘要： The present invention relates to a novel biocontrol protein which is a crystal toxin and an insecticide and which is produced in a strain of bacteria genetically altered upon incorporation of a specific gene inserted as a stable gene construction. In particular, the present invention relates to the insertion of the δ-endotoxin gene from B. thuringiensis var. tenebrionis into a cloning vector containing prokaryotic regulatory and replication systems and the utilization of this vector to transform bacterial strains into coleopteran pathogens. Limited deletion of the coding region of the gene of this invention still allows preservation of the insecticidal activity in the protein expressed. In addition, this invention provides genetically altered bacterial strains that are insect toxic and that can be used as a novel method of biological control of agricultural insect pests.

摘要翻译： 本发明涉及一种新颖的生物防治蛋白，其是结晶毒素和杀虫剂，并且是在掺入作为稳定基因构建插入的特定基因的遗传改变的细菌菌株中产生的。 特别地，本发明涉及从苏云金芽孢杆菌变种中插入δ-内毒素基因。 tenebrionis成为含有原核调节和复制系统的克隆载体，并利用该载体将细菌菌株转化为鞘翅目病原体。 本发明基因的编码区的有限缺失仍然允许保存表达的蛋白质中的杀虫活性。 此外，本发明提供了具有昆虫毒性的遗传改变的细菌菌株，可用作农业害虫生物防治的新方法。