公开(公告)号：EP3264095B1

公开(公告)日：2020-08-12

申请号：EP17181088.0

申请日：2013-01-25

申请人： Leica Biosystems Richmond, Inc.

发明人： HANSEN, Stanley, E. ,  MYERS, Russell ,  PAUL, Ronald ,  RASMUSSEN, Audra ,  TAYLOR, Kate

IPC分类号： G01N33/68

公开(公告)号：EP2807490B1

公开(公告)日：2017-09-13

申请号：EP13702719.9

申请日：2013-01-25

申请人： Leica Biosystems Richmond, Inc.

发明人： HANSEN, Stanley E. ,  MYERS, Russell ,  PAUL, Ronald ,  RASMUSSEN, Audra ,  TAYLOR, Kate

IPC分类号： G01N33/68

CPC分类号： G01N1/30 ,  G01N33/6839 ,  G01N2001/302

公开(公告)号：EP2807490A1

公开(公告)日：2014-12-03

申请号：EP13702719.9

申请日：2013-01-25

申请人： Leica Biosystems Richmond, Inc.

发明人： HANSEN, Stanley E. ,  MYERS, Russell ,  PAUL, Ronald ,  RASMUSSEN, Audra ,  TAYLOR, Kate

IPC分类号： G01N33/68

CPC分类号： G01N1/30 ,  G01N33/6839 ,  G01N2001/302

摘要： The present invention provides for solutions of a defined composition useful in a staining protocol, such as a hematoxylin and eosin staining protocol, when used at certain points of the staining protocol. The formulations of these defined solutions are such that carry-over of the solutions will not negatively impact, or preferably, will stabilize or favorably modify staining reagent solutions coming in contact with the solutions. In certain embodiments of the invention, solutions are buffered to maintain a specific pH that when carried-over-such as carried-over into hematoxylin--will not significantly influence the pH of the next staining reagent.

摘要翻译： 当在染色方案的某些点使用时，本发明提供了可用于染色方案中的限定的组合物的溶液，例如苏木精和伊红染色方案。 这些定义的溶液的制剂使得溶液的转移不会对该溶液接触的染色试剂溶液产生负面影响，或者优选地稳定或有利地修饰染色试剂溶液。 在本发明的某些实施方案中，缓冲溶液以保持特定的pH值，当被携带 - 例如携带到苏木精中时 - 不会显着影响下一个染色试剂的pH。

公开(公告)号：EP3264095A1

公开(公告)日：2018-01-03

申请号：EP17181088.0

申请日：2013-01-25

申请人： Leica Biosystems Richmond, Inc.

发明人： HANSEN, Stanley, E. ,  MYERS, Russell ,  PAUL, Ronald ,  RASMUSSEN, Audra ,  TAYLOR, Kate

IPC分类号： G01N33/68

摘要： The present invention provide for solutions of a defined composition useful in a staining protocol, such as a hematoxylin and eosin staining protocol, when used at certain points of the staining protocol. The formulations of these defined solutions are such that carry-over of the solutions will not negatively impact, or preferably, will stabilize or favorably modify staining reagent solutions coming in contact with the solutions. In certain embodiments of the invention, solutions are buffered to maintain a specific pH that when carried-over-such as carried-over into hematoxylin-will not significantly influence the pH of the next staining reagent.

摘要翻译： 当在染色方案的某些点使用时，本发明提供用于染色方案中的限定组合物的溶液，例如苏木精和曙红染色方案。 这些限定的溶液的配方使得溶液的携带不会不利地影响或优选地稳定或有利地改变与溶液接触的染色试剂溶液。 在本发明的某些实施方案中，将溶液缓冲以保持特定的pH，当其携带时（例如携带入苏木精）不会显着影响下一种染色试剂的pH。