公开(公告)号：EP2351827A1

公开(公告)日：2011-08-03

申请号：EP10175462.0

申请日：2002-03-27

申请人： Life Technologies Corporation

发明人： Ciccarone, Valentina ,  Gruber, Dale ,  Bennett, Shelly

IPC分类号： C12N1/00 ,  C12N5/00

CPC分类号： C12N9/2471 ,  C12N5/0018 ,  C12N5/0043 ,  C12N5/005 ,  C12N15/88 ,  C12N2500/22 ,  C12N2500/24 ,  C12N2501/999 ,  C12N2510/02

摘要： The present invention is directed generally to cell culture media (particularly serum free, non animal derived, and/or chemically defined media) which are useful for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells). According to the invention, such introduction can take place in the presence of said medium. Cells containing such introduced materials can then be cultured in the medium and the effect of the introduced materials on the cells can be measured or determined. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly eukaryotic cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention thus provides efficient and high throughput methods to transform/transfect culture and cells avoiding the need for multiple manipulations and transfers of cells during transfection and expression studies. The invention also relates to compositions and kits useful for culturing and transforming/transfecting cells.

摘要翻译： 本发明一般涉及可用于将大分子和化合物（例如，核酸分子）引入细胞（例如真核细胞）中的细胞培养基（特别是无血清，非动物衍生和/或化学界定的培养基）。 根据本发明，这种引入可以在所述介质的存在下进行。 然后可以在培养基中培养含有这种引入的物质的细胞，并且可以测量或测定引入的细胞对细胞的影响。 特别地，本发明允许将核酸分子（例如，载体）引入细胞（特别是真核细胞）中，以及由核酸分子在细胞中表达的蛋白质的表达。 本发明消除了在每次用细胞（例如，培养细胞与转染细胞）进行不同程序时改变细胞培养基的需要。 因此，本发明提供了有效和高通量的方法来转化/转染培养物和细胞，避免了在转染和表达研究期间对细胞的多次操作和转移的需要。 本发明还涉及可用于培养和转化/转染细胞的组合物和试剂盒。