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公开(公告)号：EP2839029B1
公开(公告)日：2017-11-15
申请号：EP13719209.2
申请日：2013-04-19
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/6851 , C12Q1/6858 , C12Q2527/119 , C12Q2563/159 , C12Q2565/607 , C12Q2565/301
摘要： A method of detection of a target nucleic acid is provided. The method includes fractionating a sample into a plurality of sample volumes wherein more than 50% of the fractions contain no more than 1 target nucleic acid molecule per sample volumes, and subjecting the plurality of sample volumes to conditions for amplification. The method further includes detecting a change in ion concentration in a sample volume wherein a target nucleic acid is present, counting the number of fractions with an amplified target nucleic acid, and determining the quantity of target nucleic acid in the sample.
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2.发明公开UNIVERSAL REPORTER-BASED GENOTYPING METHODS AND MATERIALS 有权VERFAHREN UND MATERIALIENFÜRUNIVERSELLE REPORTERBASIERTE GENOTYPISIERUNG
公开(公告)号：EP2971095A2
公开(公告)日：2016-01-20
申请号：EP14717926.1
申请日：2014-03-12
发明人： LI, Kelly , DONG, Shoulian , CHEN, Caifu
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6858 , C12Q2525/155 , C12Q2525/186 , C12Q2525/301 , C12Q2565/1015
摘要： The present disclosure is drawn to methods for detection, quantitation and analysis of nucleotides of interest, for example SNPs, in nucleic acid sequences of interest using universal FRET-based reporter primers.
摘要翻译： 本公开内容涉及使用通用的基于FRET的报道引物在感兴趣的核酸序列中检测，定量和分析目标核苷酸（例如SNP）的方法。
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公开(公告)号：EP2839029A1
公开(公告)日：2015-02-25
申请号：EP13719209.2
申请日：2013-04-19
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/6851 , C12Q1/6858 , C12Q2527/119 , C12Q2563/159 , C12Q2565/607 , C12Q2565/301
摘要： A method of detection of a target nucleic acid is provided. The method includes fractionating a sample into a plurality of sample volumes wherein more than 50% of the fractions contain no more than 1 target nucleic acid molecule per sample volumes, and subjecting the plurality of sample volumes to conditions for amplification. The method further includes detecting a change in ion concentration in a sample volume wherein a target nucleic acid is present, counting the number of fractions with an amplified target nucleic acid, and determining the quantity of target nucleic acid in the sample.
摘要翻译： 提供了靶核酸的检测方法。 该方法包括将样品分馏成多个样品体积，其中超过50％的级分每个样品体积含有不超过1个靶核酸分子，并且对多个样品体积进行扩增条件。 该方法还包括检测其中存在靶核酸的样品体积中的离子浓度的变化，用扩增的靶核酸计数分数，并测定样品中靶核酸的量。
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公开(公告)号：EP2411543A2
公开(公告)日：2012-02-01
申请号：EP10756973.3
申请日：2010-03-26
发明人： CHEN, Caifu , TAN, Ruoying
CPC分类号： C12Q1/6858 , C12Q1/686 , C12Q1/6883 , C12Q2600/156 , C12Q2561/113 , C12Q2561/101 , C12Q2537/161 , C12Q2525/186 , C12Q2565/107 , C12Q2535/125
摘要： In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as
c ompetitive
a llele-
s pecific
T aqMan PCR ("cast-PCR").-
公开(公告)号：EP2971095B1
公开(公告)日：2019-11-20
申请号：EP14717926.1
申请日：2014-03-12
发明人： LI, Kelly , DONG, Shoulian , CHEN, Caifu
IPC分类号： C12Q1/6858
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6.发明公开SMALL RNA CAPTURE, DETECTION AND QUANTIFICATION 有权ERFASSUNG，DETEKTION UND QUANTIFIZIERUNG VON KLEINER RNA
公开(公告)号：EP2914743A1
公开(公告)日：2015-09-09
申请号：EP13795633.0
申请日：2013-11-04
发明人： WONG, Linda , CHEN, Caifu , WU, Yalei , DONG, Shoulian , LIU, Chunmei
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6855 , C12P19/34 , C12Q1/6832 , C12Q1/6848 , C12Q1/6851 , C12Q1/6853 , C12Q1/6858 , C12Q1/686 , C12Q2525/191 , C12Q2525/301
摘要： The present disclosure provides novel primers and method for the detection of specific nucleic acid sequences. The primers and methods provided herein are useful in a wide variety of molecular biology applications and are particularly useful in allele-specific PCR.
摘要翻译： 本公开提供了用于检测特异性核酸序列的新型引物和方法。 本文提供的引物和方法可用于各种分子生物学应用，并且特别适用于等位基因特异性PCR。
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公开(公告)号：EP2914743B1
公开(公告)日：2019-08-07
申请号：EP13795633.0
申请日：2013-11-04
发明人： WONG, Linda , CHEN, Caifu , WU, Yalei , DONG, Shoulian , LIU, Chunmei
IPC分类号： C12Q1/6851 , C12P19/34 , C12Q1/6832 , C12Q1/6853 , C12Q1/6855 , C12Q1/6858
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公开(公告)号：EP2569452B1
公开(公告)日：2020-03-25
申请号：EP11781377.4
申请日：2011-05-13
发明人： BROOMER, Adam , LI, Kelly , TOBLER, Andreas , CHEN, Caifu , KEYS, David, Jr.
IPC分类号： C12Q1/6844
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公开(公告)号：EP2411543B1
公开(公告)日：2017-04-19
申请号：EP10756973.3
申请日：2010-03-26
发明人： CHEN, Caifu , TAN, Ruoying
CPC分类号： C12Q1/6858 , C12Q1/686 , C12Q1/6883 , C12Q2600/156 , C12Q2561/113 , C12Q2561/101 , C12Q2537/161 , C12Q2525/186 , C12Q2565/107 , C12Q2535/125
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公开(公告)号：EP2376659B1
公开(公告)日：2015-12-02
申请号：EP09836510.9
申请日：2009-12-17
发明人： CHEN, Caifu , TAN, Ruoying
CPC分类号： C12Q1/6886 , C07H21/04 , C12Q1/6858 , C12Q2600/156 , C12Q2600/172 , C12Q2561/113 , C12Q2561/101 , C12Q2537/161
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