-
1.发明公开Method for producing a polypeptide in vitro in mammalian cells in a protein-free and serum-free culture medium 失效用于在培养基中的哺乳动物细胞在体外生产多肽无血清和无蛋白质的方法
公开(公告)号：EP2221361A2
公开(公告)日：2010-08-25
申请号：EP10154450.0
申请日：1997-09-02
发明人： Gorfien, Stephen F. , Fike, Richard M. , Dzimian, Joyce L. , Godwin, Glenn P. , Price, Paul J. , Epstein, David A. , Gruber, Dale , McClure, Don
CPC分类号： C12N5/0682 , C07K14/61 , C12N5/0037 , C12N5/0043 , C12N5/005 , C12N5/0603 , C12N7/00 , C12N9/2402 , C12N9/2471 , C12N15/85 , C12N2500/14 , C12N2500/16 , C12N2500/22 , C12N2500/24 , C12N2500/32 , C12N2500/34 , C12N2500/35 , C12N2500/38 , C12N2500/74 , C12N2500/76 , C12N2500/90 , C12N2500/95 , C12N2501/90 , C12N2501/91 , C12N2710/10051 , C12Y302/01023
摘要： The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro, using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.
摘要翻译： 本发明提供细胞培养基配方确实支持在体外培养，特别是在悬浮液中，哺乳动物细胞，特别是上皮细胞和成纤维细胞，并在体外培养在悬浮液中的哺乳动物细胞，使用这些媒体方法。 的介质包含基础培养基和聚阴离子或聚阴离子化合物，优选多磺化或多硫酸化化合物，并且更优选硫酸葡聚糖。 本发明因此提供了在化学上定义的，无蛋白质的真核细胞培养介质，其包含铁螯合物和锌，所有这些是能够支持生长（特别是哺乳动物细胞的高密度生长）在悬浮培养中，增加表达的水平 的重组蛋白在培养细胞中，和/或增加病毒产生在培养的细胞。
-
公开(公告)号：EP1482031B1
公开(公告)日：2015-10-28
申请号：EP04015156.5
申请日：1997-09-02
发明人： Gorfien, Stephen F. , Fike, Richard M. , Dzimian, Joyce L. , Godwin, Glenn P. , Price, Paul J. , Epstein, David A. , Gruber, Dale , McClure, Don
CPC分类号： C12N5/0682 , C07K14/61 , C12N5/0037 , C12N5/0043 , C12N5/005 , C12N5/0603 , C12N7/00 , C12N9/2402 , C12N9/2471 , C12N15/85 , C12N2500/14 , C12N2500/16 , C12N2500/22 , C12N2500/24 , C12N2500/32 , C12N2500/34 , C12N2500/35 , C12N2500/38 , C12N2500/74 , C12N2500/76 , C12N2500/90 , C12N2500/95 , C12N2501/90 , C12N2501/91 , C12N2710/10051 , C12Y302/01023
-
3.发明公开Method for producing a polypeptide in vitro in mammalian cells in a protein-free and serum-free culture medium 失效用于在培养基中的哺乳动物细胞在体外生产多肽无血清和无蛋白质的方法
公开(公告)号：EP2221361A3
公开(公告)日：2011-02-09
申请号：EP10154450.0
申请日：1997-09-02
发明人： Gorfien, Stephen F. , Fike, Richard M. , Dzimian, Joyce L. , Godwin, Glenn P. , Price, Paul J. , Epstein, David A. , Gruber, Dale , McClure, Don
CPC分类号： C12N5/0682 , C07K14/61 , C12N5/0037 , C12N5/0043 , C12N5/005 , C12N5/0603 , C12N7/00 , C12N9/2402 , C12N9/2471 , C12N15/85 , C12N2500/14 , C12N2500/16 , C12N2500/22 , C12N2500/24 , C12N2500/32 , C12N2500/34 , C12N2500/35 , C12N2500/38 , C12N2500/74 , C12N2500/76 , C12N2500/90 , C12N2500/95 , C12N2501/90 , C12N2501/91 , C12N2710/10051 , C12Y302/01023
摘要： The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro, using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.
-
-
搜索结果
3 条记录 (耗时 0.011 秒)
