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公开(公告)号：EP2158328A2
公开(公告)日：2010-03-03
申请号：EP08755796.3
申请日：2008-05-16
CPC分类号： G01N33/582 , B33Y80/00 , G01N33/6803 , G01N2550/00
摘要： The present invention provides methods and compositions for labeling, separating and analyzing proteins, particularly a specific protein of interest within a cell lysate or in a mixture of proteins. The proteins are labeled with an amine reactive or thiol reactive fluorescent dye, or an amine reactive fluorogenic reagent that becomes fluorescent upon reacting to amine groups located on the protein. Following the labeling step, the proteins within the mixture can be separated and analyzed. In a further embodiment, a tag binding fluorogenic reagent that can bind to a tag on a tagged protein is added to specifically label the protein of interest.
摘要翻译： 本发明提供用于标记，分离和分析蛋白质，特别是细胞裂解物或蛋白质混合物中的特定目的蛋白质的方法和组合物。 蛋白质用胺反应性或巯基反应性荧光染料或胺反应性荧光试剂标记，该试剂在与位于蛋白质上的胺基反应后变成荧光。 在标记步骤之后，可以分离和分析混合物内的蛋白质。 在另一个实施方案中，加入可与标签蛋白上的标签结合的标签结合荧光试剂以特异性标记感兴趣的蛋白质。
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公开(公告)号：EP2362912A1
公开(公告)日：2011-09-07
申请号：EP09850164.6
申请日：2009-11-13
发明人： LAKSHMIPATHY, Uma , THYAGARAJAN, Bhaskar , CHESNUT, Jonathan , ANEST, Vasiliki , BENNETT, Robert , LIEU, Pauline , HANSON, George , THOMPSON, David , CHASE, Lucas , SHIPLEY, Gary , WILSON, Elizabeth
IPC分类号： C12N15/86
CPC分类号： C12N15/86 , C12N2710/16221 , C12N2710/16222 , C12N2710/16243 , C12N2799/022 , C12N2799/026 , C12N2799/027 , C12N2800/108 , C12N2820/002 , C12N2820/007 , C12N2820/60
摘要： The disclosure relates generally to genetic manipulation of stem and primary cells and to reprogramming of somatic cells, more specifically, human cells. In particular, compositions and methods are disclosed for the generation and maintenance of such engineered cells.
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公开(公告)号：EP2158328B1
公开(公告)日：2014-01-01
申请号：EP08755796.3
申请日：2008-05-16
IPC分类号： G01N33/58 , G01N33/533 , G01N33/68
CPC分类号： G01N33/582 , B33Y80/00 , G01N33/6803 , G01N2550/00
摘要： The present invention provides methods and compositions for labeling, separating and analyzing proteins, particularly a specific protein of interest within a cell lysate or in a mixture of proteins. The proteins are labeled with an amine reactive or thiol reactive fluorescent dye, or an amine reactive fluorogenic reagent that becomes fluorescent upon reacting to amine groups located on the protein. Following the labeling step, the proteins within the mixture can be separated and analyzed. In a further embodiment, a tag binding fluorogenic reagent that can bind to a tag on a tagged protein is added to specifically label the protein of interest.
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