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1.发明公开ALKYLENE GLYCOLS AND POLYMERS AND COPOLYMERS THEREOF FOR DIRECT ISOLATION OF NUCLEIC ACID FROM EMBEDDED SAMPLES 有权二醇和聚合物和共聚物及其用于从包埋的样品直接分离核酸
公开(公告)号：EP2646551A1
公开(公告)日：2013-10-09
申请号：EP11797080.6
申请日：2011-11-30
CPC分类号： C12N15/1003 , C12Q1/6804 , C12Q2527/125 , C12Q2565/518
摘要： Methods of directly isolating nucleic acid from an embedded biological sample are provided. An emulsified digest is generated in the presence of a thermostable protease, and an additive selected from an alkylene glycol, a poly(alkylene glycol), or a block copolymer having an average Mn of 76 to 2900, or a salt or derivative or combination thereof. Nucleic acid is isolated directly from the emulsified digest. The methods eliminate the use of organic solvents such as xylene in a deparaffmization step prior to isol- ating nucleic acids from paraffm-embedded samples, for example.
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2.发明授权ALKYLENE GLYCOLS AND POLYMERS AND COPOLYMERS THEREOF FOR DIRECT ISOLATION OF NUCLEIC ACID FROM EMBEDDED SAMPLES 有权二醇和聚合物和共聚物及其用于从包埋的样品直接分离核酸
公开(公告)号：EP2646551B1
公开(公告)日：2017-06-07
申请号：EP11797080.6
申请日：2011-11-30
CPC分类号： C12N15/1003 , C12Q1/6804 , C12Q2527/125 , C12Q2565/518
摘要： Methods of directly isolating nucleic acid from an embedded biological sample are provided. An emulsified digest is generated in the presence of a thermostable protease, and an additive selected from an alkylene glycol, a poly(alkylene glycol), or a block copolymer having an average Mn of 76 to 2900, or a salt or derivative or combination thereof. Nucleic acid is isolated directly from the emulsified digest. The methods eliminate the use of organic solvents such as xylene in a deparaffmization step prior to isol- ating nucleic acids from paraffm-embedded samples, for example.
摘要翻译： 直接提供分离核酸从包埋的生物样品的方法。 乳化的摘要是在热塑性表蛋白酶的存在而产生，并且添加剂从亚烷基二醇，聚（亚烷基二醇），或具有在76平均M n 2900的嵌段共聚物，或者其盐或衍生物或它们的组合中选择 ， 核酸从乳化消化直接分离。 方法消除了使用有机溶剂：例如在隔离石蜡包埋的样品核酸，例如之前的脱石蜡工序二甲苯。
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