公开(公告)号：EP1012323A1

公开(公告)日：2000-06-28

申请号：EP98903871.6

申请日：1998-01-30

申请人： Lockheed Martin Energy Research Corporation ,  UNIVERSITY OF CHICAGO

发明人： NGHIEM, Nhuan, Phu ,  DONNELLY, Mark ,  MILLARD, Cynthia, S. ,  STOLS, Lucy

IPC分类号： C12P7/40 ,  C12P7/54

CPC分类号： C12P7/44 ,  C12N9/0006 ,  C12P7/40 ,  C12P7/46 ,  C12R1/19 ,  Y02E50/17 ,  Y10S435/849 ,  Y10S435/853

摘要： The present invention is an economical fermentation method for the production of carboxylic acids comprising the steps of: a) inoculating a medium having a carbon source with a carboxylic acid-producing organism; b) incubating the carboxylic acid-producing organism in an aerobic atmosphere to promote rapid growth of the organism thereby increasing the biomass of the organism; c) controllably releasing oxygen to maintain the aerobic atmosphere; d) controllably feeding the organism having increased biomass with a solution containing the carbon source to maintain the concentration of the carbon source within the medium of about 0.5 g/L up to about 1 g/L; e) depriving the aerobic atmosphere of oxygen to produce an anaerobic atmosphere to cause the organism to undergo anaerobic metabolism; f) controllably feeding the organism having increased biomass a solution containing the carbon source to maintain the concentration of the carbon source within the medium ≥1 g/L; and g) converting the carbon source to carboxylic acids using the anaerobic metabolism of the organism.

公开(公告)号：EP1751292A1

公开(公告)日：2007-02-14

申请号：EP04751134.0

申请日：2004-05-03

申请人： UT-Battelle, LLC ,  The University of Chicago

发明人： NGHIEM, Nhuan, Phu ,  DONNELLY, Mark ,  SANVILLE-MILLARD, Cynthia, Y.

IPC分类号： C12P1/00 ,  C12P7/00 ,  C12P7/64 ,  C12P7/46 ,  C12N1/20

CPC分类号： C12N9/0006 ,  C12N9/1029 ,  C12P7/46

摘要： A method for producing succinic acid from industrial-grade hydrolysates uses an organism that contains mutations for the genes ptsG, pflB, and ldhA, and allows the organism to accumulate biomass, and allow s the organism to metabolize the hydrolysate. A bacteria mutant produces succinic acid from substrate contained in industrial-grade hydro-lysate in a ratio of between 0.6:1 and 1.3:1 succinic acid to substrate.

公开(公告)号：EP0953037A1

公开(公告)日：1999-11-03

申请号：EP96938756.0

申请日：1996-10-31

申请人： UNIVERSITY OF CHICAGO

发明人： DONNELLY, Mark ,  MILLARD, Cynthia, S. ,  STOLS, Lucy

IPC分类号： C12N15 ,  C12N1 ,  C12N9 ,  C12P7 ,  C12R1

CPC分类号： C12N9/0006 ,  C12P7/40 ,  C12P7/44 ,  C12P7/46 ,  C12R1/19 ,  Y10S435/849

摘要： A method for isolating succinic acid producing bacteria is provided comprising increasing the biomass of an organism which lacks the ability of catabolize pyruvate, and then subjecting the biomass to glucose-rich medium in an anaerobic environment to enable pyruvate-catabolizing mutants to grow. The invention also provides for a mutant that produces high amounts of succinic acid, which has been derived from a parent which lacked the genes for pyruvate formate lyase and lactate dehydrogenase, and which belongs to the E.coli Group of Bacteria.