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公开(公告)号：EP0953037A1
公开(公告)日：1999-11-03
申请号：EP96938756.0
申请日：1996-10-31
CPC分类号： C12N9/0006 , C12P7/40 , C12P7/44 , C12P7/46 , C12R1/19 , Y10S435/849
摘要： A method for isolating succinic acid producing bacteria is provided comprising increasing the biomass of an organism which lacks the ability of catabolize pyruvate, and then subjecting the biomass to glucose-rich medium in an anaerobic environment to enable pyruvate-catabolizing mutants to grow. The invention also provides for a mutant that produces high amounts of succinic acid, which has been derived from a parent which lacked the genes for pyruvate formate lyase and lactate dehydrogenase, and which belongs to the E.coli Group of Bacteria.
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公开(公告)号：EP1012323A1
公开(公告)日：2000-06-28
申请号：EP98903871.6
申请日：1998-01-30
CPC分类号： C12P7/44 , C12N9/0006 , C12P7/40 , C12P7/46 , C12R1/19 , Y02E50/17 , Y10S435/849 , Y10S435/853
摘要： The present invention is an economical fermentation method for the production of carboxylic acids comprising the steps of: a) inoculating a medium having a carbon source with a carboxylic acid-producing organism; b) incubating the carboxylic acid-producing organism in an aerobic atmosphere to promote rapid growth of the organism thereby increasing the biomass of the organism; c) controllably releasing oxygen to maintain the aerobic atmosphere; d) controllably feeding the organism having increased biomass with a solution containing the carbon source to maintain the concentration of the carbon source within the medium of about 0.5 g/L up to about 1 g/L; e) depriving the aerobic atmosphere of oxygen to produce an anaerobic atmosphere to cause the organism to undergo anaerobic metabolism; f) controllably feeding the organism having increased biomass a solution containing the carbon source to maintain the concentration of the carbon source within the medium ≥1 g/L; and g) converting the carbon source to carboxylic acids using the anaerobic metabolism of the organism.
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