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公开(公告)号：EP2940120A1
公开(公告)日：2015-11-04
申请号：EP14166918.4
申请日：2014-05-02
申请人： Lonza Cologne GmbH
CPC分类号： C12N13/00 , A61N1/0412 , C12M35/02 , C12N15/87
摘要： The invention relates to a device 1 for applying an electric field to a suspension of cells, cell derivatives, organelles, sub-cellular particles and/or vesicles, comprising at least one chamber 6 which comprises at least two electrodes 4, 5 and at least one separating element 13 which is movable within the chamber 6 between two terminal points 14, 15 and, if it is in a position between the terminal points 14, 15, separates at least one first compartment 26 of the chamber 6 from at least one second compartment 27 of the chamber 6. According to the invention each compartment 26, 27 is designed to hold the suspension and comprises at least one port 7, 8, 10, 11 for charging or discharging the suspension, so as to discharge an aliquot of the suspension from the chamber 6 and at the same time charge a further aliquot of the suspension into the chamber 6, wherein the separating element 13 is moved in a second direction opposite to a first direction, and wherein the separating element 13 separates the aliquots from each other.
摘要翻译： 本发明涉及用于将电场施加到细胞，细胞衍生物，细胞器，亚细胞颗粒和/或囊泡的悬浮液的装置1，其包括至少一个腔室6，腔室6包括至少两个电极4,5，并且至少 一个分离元件13在腔室6内在两个终点14,15之间可移动，并且如果它处于终点14,15之间的位置，则将腔室6的至少一个第一隔室26与至少一个第二 根据本发明，每个隔室26,27被设计成容纳悬浮液并且包括至少一个用于充入或排出悬浮液的端口7,8,10,11，以便将等分部分的 从室6悬浮，同时将另一等份悬浮液装入室6中，其中分离元件13在与第一方向相反的第二方向上移动，并且其中分离元件13 激起对方的等分。
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2.发明公开
公开(公告)号：EP2208780A8
公开(公告)日：2012-06-20
申请号：EP09000701.4
申请日：2009-01-20
申请人： Lonza Cologne GmbH
发明人： Altrogge, Ludger , Gleißner, Timo , Heinze, Andreas , Müller-Hartmann, Herbert , Wirth, Andreas
CPC分类号： C12M35/02 , C12M23/44 , Y10T29/49117
摘要： Die Erfindung betrifft ein Verfahren zur Beaufschlagung von mindestens zwei mit Elektroden versehenen Behältnissen mit mindestens einem Spannungsimpuls, bei dem mindestens ein Behältnis mit mindestens einem Spannungsimpuls beaufschlagt wird, während mindestens ein anderes Behältnis einer Vor- oder Nachbereitung unterzogen wird. Erfindungsgemäß umfasst das Verfahren das gegenseitiges Tauschen der jeweiligen Positionen eines bereits mit mindestens einem Spannungsimpuls beaufschlagten Behältnisses und eines weiteren Behältnisses. Die Erfindung betrifft ferner eine Vorrichtung 1 zur elektrischen Kontaktierung mindestens eines mit Elektroden versehenen Behältnisses, mit mindestens einer Aufnahmevorrichtung 3, 7 zum Aufsetzen mindestens eines Behältnisses und mit mindestens einer Kontakteinrichtung 8 zur Kontaktierung der Elektroden des Behältnisses. Erfindungsgemäß sind mindestens zwei Aufnahmevorrichtungen 3, 7 vorgesehen, wobei sich eine Aufnahmevorrichtung 7 an oder innerhalb der Kontakteinrichtung 8 befindet und wobei beide Aufnahmevorrichtungen 3, 7 und/oder die Kontakteinrichtung 8 derart bewegbar sind, dass sich die andere Aufnahmevorrichtung 3 nach Abschluss der Bewegung an oder innerhalb der Kontakteinrichtung 8 befindet.
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公开(公告)号：EP2940121B1
公开(公告)日：2018-08-01
申请号：EP14191272.5
申请日：2014-10-31
申请人： Lonza Cologne GmbH
CPC分类号： C12N13/00 , A61N1/0412 , C12M35/02 , C12N15/87
摘要： The invention relates to a device for applying an electric field to a suspension of cells, comprising at least one chamber which comprises at least one internal space 40 for holding the suspension, the internal space 40 comprising at least two segments 41, 42, wherein each segment 41, 42 comprises at least one electrode 43, 44 and wherein neighboring electrodes 43, 44 are separated from each other by at least one gap 47 which is at least partially filled with an insulating material 46, and wherein the edges of the electrodes 43, 44 facing each other within the internal space 40 are rounded. Rounding the electrodes' edges facing a neighboring electrode results in a significant reduction of field gradients and thus even of the risk of arcing. The invention further concerns a method, wherein voltage is applied to at least one active electrode 43, 44 while the electrodes 43, 44, 45 or electrode segments next and/or opposite to the active electrode 43, 44 are set to ground potential. Setting neighboring electrodes that surround the active electrode to ground potential results in decreased scattering of the electric field within the internal space so that the electrically active area is locally limited and the field lines are focused near the active electrode and thus control of the process is enhanced.
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公开(公告)号：EP3260163A1
公开(公告)日：2017-12-27
申请号：EP17184306.3
申请日：2014-05-02
申请人： Lonza Cologne GmbH
CPC分类号： C12N13/00 , A61N1/0412 , C12M35/02 , C12N15/87
摘要： The invention relates to a device 1 for applying an electric field to a suspension of cells, cell derivatives, organelles, sub-cellular particles and/or vesicles, comprising at least one chamber 6 which comprises at least two segments, each segment comprising at least one electrode 4, 5, wherein at least one port 7, 8 is disposed at one end 9 of the chamber 6 and at least one further port 10, 11 is disposed at the opposite end 12 of the chamber 6. According to the invention each segment is provided with at least one first electrode 4 and at least one second electrode 5, wherein the second electrode 5 is a common electrode of at least two segments. In order to avoid arcing and/or undesired heating of the suspension, voltage pulses can be applied to different segments of the chamber sequentially.
摘要翻译： 本发明涉及一种用于向细胞，细胞衍生物，细胞器，亚细胞颗粒和/或囊泡的悬浮液施加电场的装置1，其包括至少一个腔室6，腔室6包括至少两个区段，每个区段至少包括 一个电极4,5，其中至少一个端口7,8设置在腔室6的一个端部9处，并且至少一个另外的端口10,11设置在腔室6的相对端部12处。根据本发明，每个 段设置有至少一个第一电极4和至少一个第二电极5，其中第二电极5是至少两段的公共电极。 为了避免悬浮的电弧和/或不希望的加热，可以将电压脉冲顺序地施加到腔室的不同区段。
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公开(公告)号：EP2940120B1
公开(公告)日：2018-08-01
申请号：EP14166918.4
申请日：2014-05-02
申请人： Lonza Cologne GmbH
CPC分类号： C12N13/00 , A61N1/0412 , C12M35/02 , C12N15/87
摘要： The invention relates to a device for applying an electric field to a suspension of cells, comprising at least one chamber which comprises at least one internal space 40 for holding the suspension, the internal space 40 comprising at least two segments 41, 42, wherein each segment 41, 42 comprises at least one electrode 43, 44 and wherein neighboring electrodes 43, 44 are separated from each other by at least one gap 47 which is at least partially filled with an insulating material 46, and wherein the edges of the electrodes 43, 44 facing each other within the internal space 40 are rounded. Rounding the electrodes' edges facing a neighboring electrode results in a significant reduction of field gradients and thus even of the risk of arcing. The invention further concerns a method, wherein voltage is applied to at least one active electrode 43, 44 while the electrodes 43, 44, 45 or electrode segments next and/or opposite to the active electrode 43, 44 are set to ground potential. Setting neighboring electrodes that surround the active electrode to ground potential results in decreased scattering of the electric field within the internal space so that the electrically active area is locally limited and the field lines are focused near the active electrode and thus control of the process is enhanced.
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6.发明授权
公开(公告)号：EP2208780B1
公开(公告)日：2017-11-01
申请号：EP09000701.4
申请日：2009-01-20
申请人： Lonza Cologne GmbH
发明人： Altrogge, Ludger , Gleißner, Timo , Heinze, Andreas , Müller-Hartmann, Herbert , Wirth, Andreas
CPC分类号： C12M35/02 , C12M23/44 , Y10T29/49117
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7.发明公开Device and method for large volume transfection 审中-公开Vorrichtung und VerfahrenfürgroßvolumigeTransfektion
公开(公告)号：EP2940121A1
公开(公告)日：2015-11-04
申请号：EP14191272.5
申请日：2014-10-31
申请人： Lonza Cologne GmbH
CPC分类号： C12N13/00 , A61N1/0412 , C12M35/02 , C12N15/87
摘要： The invention relates to a device for applying an electric field to a suspension of cells, comprising at least one chamber which comprises at least one internal space 40 for holding the suspension, the internal space 40 comprising at least two segments 41, 42, wherein each segment 41, 42 comprises at least one electrode 43, 44 and wherein neighboring electrodes 43, 44 are separated from each other by at least one gap 47 which is at least partially filled with an insulating material 46, and wherein the edges of the electrodes 43, 44 facing each other within the internal space 40 are rounded. Rounding the electrodes' edges facing a neighboring electrode results in a significant reduction of field gradients and thus even of the risk of arcing. The invention further concerns a method, wherein voltage is applied to at least one active electrode 43, 44 while the electrodes 43, 44, 45 or electrode segments next and/or opposite to the active electrode 43, 44 are set to ground potential. Setting neighboring electrodes that surround the active electrode to ground potential results in decreased scattering of the electric field within the internal space so that the electrically active area is locally limited and the field lines are focused near the active electrode and thus control of the process is enhanced.
摘要翻译： 本发明涉及一种用于向电池悬浮液施加电场的装置，包括至少一个腔室，该腔室包括用于保持悬架的至少一个内部空间40，内部空间40包括至少两个区段41,42，其中每个腔室 段41,42包括至少一个电极43,44，并且其中相邻电极43,44通过至少一部分填充有绝缘材料46的间隙47彼此分开，并且其中电极43的边缘 在内部空间40内彼此面对的四边形。 将电极的边缘朝向邻近的电极，导致现场梯度的显着降低，从而导致电弧的风险。 本发明还涉及一种方法，其中将电压施加到至少一个有源电极43,44，而将电极43,44,45或与有源电极43,44相邻和/或相对的电极段设置为接地电位。 将围绕有源电极的相邻电极设置为接地电位导致电场在内部空间内的散射减少，使得电活动区域局部受限并且场线聚焦在有源电极附近，从而增强了该过程的控制 。
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9.发明授权Behältnis mit mehreren Reaktionsräumen und Elektroden 有权Behältnismit mehrerenReaktionsräumenund Elektroden
公开(公告)号：EP2208779B1
公开(公告)日：2017-04-05
申请号：EP09000700.6
申请日：2009-01-20
申请人： Lonza Cologne GmbH
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