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公开(公告)号：EP3402496A1
公开(公告)日：2018-11-21
申请号：EP17738957.4
申请日：2017-01-12
IPC分类号： A61K35/545 , C12N5/00 , C12N5/04 , C12N15/63
CPC分类号： C12N5/0696 , A61K35/545 , C07K14/005 , C07K14/4702 , C12N9/1211 , C12N9/78 , C12N15/85 , C12N2501/60 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/605 , C12N2501/606 , C12N2501/608 , C12N2506/11 , C12N2510/00 , C12N2710/16231 , C12N2800/108 , C12N2820/60 , C12N2830/003 , C12N2830/006 , C12Y207/01021 , C12Y305/04001
摘要： The invention relates generally to methods of generating induced pluripotent stem cells (iPSCs) that do not contain the reprogramming vector. In some embodiments, the invention relates to inducing pluripotency in somatic cells by introducing an episomal vector(s) comprising at least one expression cassette containing reprogramming factors and/or synthetic transcription factors and a suicide gene. In some embodiments, the invention relates to inducing pluripotency in somatic cells by introducing episomal vector(s) comprising expression cassettes containing reprogramming factors and/or synthetic transcription factors and a transcriptionally regulated EBNA-1 gene. In some embodiments, the invention relates to inducing pluripotency in somatic cells by introducing episomal vector(s) comprising expression cassettes containing reprogramming factors and/or synthetic transcription factors and both a suicide gene and a transcriptionally regulated EBNA-1 gene.
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2.发明公开
公开(公告)号：EP3307893A1
公开(公告)日：2018-04-18
申请号：EP16808495.2
申请日：2016-06-13
CPC分类号： C12N15/85 , A61K35/12 , A61K35/15 , A61K35/33 , A61K35/51 , C12N5/0696 , C12N2501/40 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2501/608 , C12N2506/11 , C12N2510/00 , G01N33/5073
摘要： The current disclosure provides methods for reprogramming mammalian somatic cells by regulating the expression of endogenous cellular genes. Cellular reprogramming of somatic cells can be induced by activating the transcription of embryonic stem cell-associated genes (e.g., oct3/4) and suppressing the transcription of somatic cell-specific and/or cell death-associated genes. The endogenous transcription machinery can be modulated using synthetic transcription factors (activators and suppressors), to allow for faster, and more efficient nuclear reprogramming under conditions amenable for clinical and commercial applications. The current disclosure further provides cells obtained from such methods, along with therapeutic methods for using such cells for the treatment of diseases amendable to stem cell therapy, as well as kits for such uses.
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