公开(公告)号：EP0206792B1

公开(公告)日：1991-11-06

申请号：EP86304799.9

申请日：1986-06-23

申请人： MASSEY UNIVERSITY

发明人： Poll, Dick Jacob ,  Harding, David Roger Kay ,  Hancock, William Stephan

IPC分类号： C07K3/20 ,  B01D15/08

CPC分类号： C07K1/16 ,  B01D15/426

公开(公告)号：EP0206792A3

公开(公告)日：1987-06-10

申请号：EP86304799

申请日：1986-06-23

申请人： MASSEY UNIVERSITY

发明人： Poll, Dick Jacob ,  Harding, David Roger Kay ,  Hancock, William Stephan

IPC分类号： C07K03/20 ,  B01D15/08

CPC分类号： C07K1/16 ,  B01D15/426

摘要： There is described a mobile phase for use in the purification of proteins using reversed phase HPLC on a preparative scale. The mobile phase is an aqueous solution containing .005 to 1 M of a physiologically acceptable acid of the general formula YCOOH where Y is a polar electron withdrawing group capable of hydrogen bonding silanol groups. The solution also contains up to 95% V of a physiologically acceptable organic solvent. A preparative method of purifying proteins such as HSA or BSA using reversed phase HPLC is also described.