公开(公告)号：EP0332879A3

公开(公告)日：1991-05-02

申请号：EP89102786.4

申请日：1989-02-17

申请人： MECT CORPORATION

发明人： Kannagi, Reiji ,  Kirihata, Yoshiko ,  Ogawa, Tomoya ,  Numata, Masaaki ,  Sugimoto, Mamoru

IPC分类号： C12P21/08 ,  C12P21/00 ,  C12N15/00

CPC分类号： C07K16/30 ,  A61K38/00 ,  C07K16/44

摘要： A monoclonal antibody exhibiting specificity to a sialic acid glycolipid which contains at least one epitope selected from the group consisting of NeuAc α 2 → 9NeuAc terminal, NeuAc α 2 → 6Galβ terminal and NeuAc α2 → 1Cer. A hybridoma which produces said monoclonal antibody. A process for producing such a hybridoma.

公开(公告)号：EP0332879A2

公开(公告)日：1989-09-20

申请号：EP89102786.4

申请日：1989-02-17

申请人： MECT CORPORATION

发明人： Kannagi, Reiji ,  Kirihata, Yoshiko ,  Ogawa, Tomoya ,  Numata, Masaaki ,  Sugimoto, Mamoru

IPC分类号： C12P21/08 ,  C12P21/00 ,  C12N15/00

CPC分类号： C07K16/30 ,  A61K38/00 ,  C07K16/44

摘要： A monoclonal antibody exhibiting specificity to a sialic acid glycolipid which contains at least one epitope selected from the group consisting of NeuAc α 2 → 9NeuAc terminal, NeuAc α 2 → 6Galβ terminal and NeuAc α2 → 1Cer. A hybridoma which produces said monoclonal antibody. A process for producing such a hybridoma.

摘要翻译： 对唾液酸糖脂显示特异性的单克隆抗体，其含有至少一种选自NeuAcα2-> 9NeuAc末端，NeuAcα2->6Galβ末端和NeuAcα2→1Cer的表位。 产生所述单克隆抗体的杂交瘤。 一种生产这种杂交瘤的方法。

公开(公告)号：EP0369425A1

公开(公告)日：1990-05-23

申请号：EP89121138.5

申请日：1989-11-15

申请人： MECT CORPORATION

发明人： Hino, Akihiro ,  Kannagi, Reiji ,  Suda, Isao ,  Numata, Masaaki ,  Shibayama, Shohei ,  Sugimoto, Mamoru ,  Tomita, Kenkichi ,  Ogawa, Tomoya

IPC分类号： C12P21/08 ,  C12N5/24

CPC分类号： C07K16/30 ,  A61K38/00

摘要： A monoclonal antibody having specificity to N-glycolyl type GM₂ and a hybridoma producing a monoclonal antibody which has specificity to N-glycolyl type GM₂ are described. The hybridoma can be established according to a method which comprises the fusion of (A) B-­cells obtained by immunizing an animal from which thymus is removed when it is a neonate using a synthetic N-glycolyl type GM₃ as an immunogen, and (b) myeloma cells. The monoclonal antibody can be used in, for instance, ELISA and TLC immunostaining which are usually adopted for the detection of cancer-related glycolipids.

摘要翻译： 描述了具有特异性的N-羟GM2型和杂交瘤生产其中具有特异性N-羟GM2型的单克隆抗体的单克隆抗体。 杂交瘤可以雅丁，其包含通过免疫（从哪个胸腺动物获得（A）的B细胞的融合被移除。当它是使用合成N-羟型GM3作为免疫原性新生儿的方法，和b来建立 ）骨髓瘤细胞。 的单克隆抗体可用于，例如，ELISA和免疫染色TLC其通常采用的检测与癌症相关的糖脂。