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公开(公告)号：EP1354034B8
公开(公告)日：2008-06-18
申请号：EP01987182.1
申请日：2001-11-30
发明人： TOMIZUKA, Kazuma , ISHIDA, Isao , LONBERG, Nils , HALK, Ed
IPC分类号： C12N15/00 , A01K67/027 , C12N5/06 , C07K16/00 , C12N5/20
CPC分类号： A01K67/0278 , A01K67/02 , A01K67/0276 , A01K2207/15 , A01K2217/00 , A01K2217/072 , A01K2217/075 , A01K2217/15 , A01K2217/30 , A01K2227/105 , A01K2267/01 , A61K39/00 , A61K2039/505 , C07K14/70535 , C07K16/00 , C07K16/18 , C07K16/243 , C07K16/2812 , C07K16/2818 , C07K16/2863 , C07K2317/14 , C07K2317/21 , C07K2317/24 , C07K2317/92 , C12N5/0635 , C12N5/163 , C12N15/8509 , C12N2015/8518 , C12P21/005 , C12Q1/6881 , C12Q2600/158
摘要： The present invention provides a transgenic nonhuman mammal, preferably a rodent, comprising two human immunoglobulin loci, wherein one of two said human immunoglobulin loci is a human heavy chain locus and the other locus is a human light chain locus; and wherein only one of said loci is of a transchromosome. In some transgenic nonhuman mammals, the endogenous mammalian heavy chain locus and at least one mammalian light chain locus are inactivated. In some such transgenic nonhuman mammals, the endogenous mammalian heavy chain locus and kappa light chain locus are inactivated. The present invention further provides methods of generating a plurality of B cells from a transgenic nonhuman mammal expressing human antibody sequences. Additional methods of the invention comprise fusing the plurality of B cells with immortalized cells to form hybridomas. The present invention also provides methods of generating a human sequence antibody from a transgenic nonhuman mammal.
摘要翻译： 本发明提供了包含两个人免疫球蛋白基因座的转基因非人哺乳动物，优选啮齿动物，其中两个所述人免疫球蛋白基因座中的一个是人重链基因座，另一个基因座是人轻链基因座; 并且其中只有一个所述基因座是转染色体。 在一些转基因非人类哺乳动物中，内源哺乳动物重链基因座和至少一个哺乳动物轻链基因座失活。 在一些这样的转基因非人类哺乳动物中，内源性哺乳动物重链基因座和κ轻链基因座失活。 本发明进一步提供了从表达人抗体序列的转基因非人哺乳动物产生多种B细胞的方法。 本发明的其他方法包括将多个B细胞与永生化细胞融合以形成杂交瘤。 本发明还提供了从转基因非人类哺乳动物产生人类序列抗体的方法。
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2.发明授权TRANSGENIC TRANSCHROMOSOMAL RODENTS FOR MAKING HUMAN ANTIBODIES 有权TRANSCHROMOSOMALE TRANSGEN-鼠类为制造抗体HUMÄNEN
公开(公告)号：EP1354034B1
公开(公告)日：2007-11-14
申请号：EP01987182.1
申请日：2001-11-30
发明人： TOMIZUKA, Kazuma , ISHIDA, Isao , LONBERG, Nils , HALK, Ed
IPC分类号： C12N15/00 , A01K67/027 , C12N5/06 , C07K16/00 , C12N5/20
CPC分类号： A01K67/0278 , A01K67/02 , A01K67/0276 , A01K2207/15 , A01K2217/00 , A01K2217/072 , A01K2217/075 , A01K2217/15 , A01K2217/30 , A01K2227/105 , A01K2267/01 , A61K39/00 , A61K2039/505 , C07K14/70535 , C07K16/00 , C07K16/18 , C07K16/243 , C07K16/2812 , C07K16/2818 , C07K16/2863 , C07K2317/14 , C07K2317/21 , C07K2317/24 , C07K2317/92 , C12N5/0635 , C12N5/163 , C12N15/8509 , C12N2015/8518 , C12P21/005 , C12Q1/6881 , C12Q2600/158
摘要： The present invention provides a transgenic nonhuman mammal, preferably a rodent, comprising two human immunoglobulin loci, wherein one of two said human immunoglobulin loci is a human heavy chain locus and the other locus is a human light chain locus; and wherein only one of said loci is of a transchromosome. In some transgenic nonhuman mammals, the endogenous mammalian heavy chain locus and at least one mammalian light chain locus are inactivated. In some such transgenic nonhuman mammals, the endogenous mammalian heavy chain locus and kappa light chain locus are inactivated. The present invention further provides methods of generating a plurality of B cells from a transgenic nonhuman mammal expressing human antibody sequences. Additional methods of the invention comprise fusing the plurality of B cells with immortalized cells to form hybridomas. The present invention also provides methods of generating a human sequence antibody from a transgenic nonhuman mammal.
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3.发明公开TRANSGENIC TRANSCHROMOSOMAL RODENTS FOR MAKING HUMAN ANTIBODIES 有权TRANSCHROMOSOMALE TRANSGEN-鼠类为制造抗体HUMÄNEN
公开(公告)号：EP1354034A2
公开(公告)日：2003-10-22
申请号：EP01987182.1
申请日：2001-11-30
发明人： TOMIZUKA, Kazuma , ISHIDA, Isao , LONBERG, Nils , HALK, Ed
IPC分类号： C12N15/00 , A01K67/027 , C12N5/06 , C07K16/00 , C12N5/20
CPC分类号： A01K67/0278 , A01K67/02 , A01K67/0276 , A01K2207/15 , A01K2217/00 , A01K2217/072 , A01K2217/075 , A01K2217/15 , A01K2217/30 , A01K2227/105 , A01K2267/01 , A61K39/00 , A61K2039/505 , C07K14/70535 , C07K16/00 , C07K16/18 , C07K16/243 , C07K16/2812 , C07K16/2818 , C07K16/2863 , C07K2317/14 , C07K2317/21 , C07K2317/24 , C07K2317/92 , C12N5/0635 , C12N5/163 , C12N15/8509 , C12N2015/8518 , C12P21/005 , C12Q1/6881 , C12Q2600/158
摘要： The present invention provides a transgenic nonhuman mammal, preferably a rodent, comprising two human immunoglobulin loci, wherein one of two said human immunoglobulin loci is a human heavy chain locus and the other locus is a human light chain locus; and wherein only one of said loci is of a transchromosome. In some transgenic nonhuman mammals, the endogenous mammalian heavy chain locus and at least one mammalian light chain locus are inactivated. In some such transgenic nonhuman mammals, the endogenous mammalian heavy chain locus and kappa light chain locus are inactivated. The present invention further provides methods of generating a plurality of B cells from a transgenic nonhuman mammal expressing human antibody sequences. Additional methods of the invention comprise fusing the plurality of B cells with immortalized cells to form hybridomas. The present invention also provides methods of generating a human sequence antibody from a transgenic nonhuman mammal.
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