公开(公告)号：EP1702983A3

公开(公告)日：2007-01-10

申请号：EP06001413.1

申请日：2001-04-13

申请人： MEDICAL UNIVERSITY OF SOUTH CAROLINA ,  THE PENN STATE RESEARCH FOUNDATION

发明人： Norris, James, S. ,  Clawson, Gary, A. ,  Westwater, Caroline ,  Schofield, David ,  Schmidt, Michael, G. ,  Hoel, Brian ,  Dolan, Joseph ,  Pan, Wei-Hua

IPC分类号： C12N15/11 ,  C12N15/74

CPC分类号： C12N15/113 ,  A61K38/00 ,  A61K48/00 ,  C12N15/1131 ,  C12N15/74 ,  C12N2310/111 ,  C12N2310/12 ,  C12N2310/317

摘要： The present invention relates to the discovery, identification and characterization of toxic agents which are lethal to pathogens and methods for targeting such toxic agents to a pathogen or pathogen infected cells in order to treat and/or eradicate the infection. In particular, the present invention relates to toxic agents which target bacteria at different stages of the bacterial life cycle, which are delivered alone or in combination to bacteria or bacteria-infected cells. The invention relates to toxic agents which are lethal to diseased cells and methods for targeting such toxic agents to a diseased cell in order to treat and/or eradicate the disease. The present invention relates to promoter elements which are pathogen-specific or tissue-specific and the use of such promoter elements to achieve pathogen-specific or tissue-specific expression of the toxic agent(s) and/or ribozyme(s) of the present invention. Specifically, the invention relates to the delivery of one or more toxic gene products, antisense RNAs, or ribozymes, or combination thereof. The invention provides a novel system by which multiple pathogenic targets may be simultaneously targeted to cause the death of a pathogen, or cell infected with a pathogen. Further, the invention has important implications in the eradication of drug-resistant bacterium and bacterial pathogens. The invention provides a novel system by which multiple targets may be simultaneously targeted to cause the death of a diseased cell. The invention has important implications in the eradication of drug-resistant pathogens (such as antibiotic resistant bacteria) and drug-resistant diseased cells (such as drug-resistant cancer cells).

公开(公告)号：EP1702983A2

公开(公告)日：2006-09-20

申请号：EP06001413.1

申请日：2001-04-13

申请人： MEDICAL UNIVERSITY OF SOUTH CAROLINA ,  THE PENN STATE RESEARCH FOUNDATION

发明人： Norris, James, S. ,  Clawson, Gary, A. ,  Westwater, Caroline ,  Schofield, David ,  Schmidt, Michael, G. ,  Hoel, Brian ,  Dolan, Joseph ,  Pan, Wei-Hua

IPC分类号： C12N15/11 ,  C12N15/74

CPC分类号： C12N15/113 ,  A61K38/00 ,  A61K48/00 ,  C12N15/1131 ,  C12N15/74 ,  C12N2310/111 ,  C12N2310/12 ,  C12N2310/317

摘要： The present invention relates to the discovery, identification and characterization of toxic agents which are lethal to pathogens and methods for targeting such toxic agents to a pathogen or pathogen infected cells in order to treat and/or eradicate the infection. In particular, the present invention relates to toxic agents which target bacteria at different stages of the bacterial life cycle, which are delivered alone or in combination to bacteria or bacteria-infected cells. The invention relates to toxic agents which are lethal to diseased cells and methods for targeting such toxic agents to a diseased cell in order to treat and/or eradicate the disease. The present invention relates to promoter elements which are pathogen-specific or tissue-specific and the use of such promoter elements to achieve pathogen-specific or tissue-specific expression of the toxic agent(s) and/or ribozyme(s) of the present invention. Specifically, the invention relates to the delivery of one or more toxic gene products, antisense RNAs, or ribozymes, or combination thereof. The invention provides a novel system by which multiple pathogenic targets may be simultaneously targeted to cause the death of a pathogen, or cell infected with a pathogen. Further, the invention has important implications in the eradication of drug-resistant bacterium and bacterial pathogens. The invention provides a novel system by which multiple targets may be simultaneously targeted to cause the death of a diseased cell. The invention has important implications in the eradication of drug-resistant pathogens (such as antibiotic resistant bacteria) and drug-resistant diseased cells (such as drug-resistant cancer cells).

摘要翻译： 本发明涉及到有毒试剂哪个是致命的病原体和方法以治疗和/或根除感染靶向寻求病原体或病原体感染的细胞毒性剂的发现，鉴定和表征。 特别地，本发明涉及靶向于在细菌生命周期的不同阶段，它们是单独或组合使用以细菌或细菌感染的细胞递送细菌毒性剂。 本发明涉及一种毒性剂哪个是致命的患病细胞和方法以治疗和/或根除疾病靶向测试毒性剂至患病细胞。 本发明涉及本的启动子元件，其是病原体特异性或组织特异性和使用搜索启动子元件，以实现病原体特异性或组织特异性毒性剂的表达和/或核酶（S） 发明。 具体而言，本发明涉及的一种或多种有毒基因产物，反义RNA或核酶，或者它们的组合递送。 本发明提供了一种新颖的系统，通过该多个致病目标可同时靶向引起病原体的受病原体感染的死亡或细胞。 此外，本发明具有耐药细菌和细菌病原体的根除重要的意义。 本发明提供了一种新颖的系统，通过该多个目标可同时靶向引起病变细胞的死亡。 本发明已经在耐药病原体的根除重要影响（：如抗生素抗性细菌）和耐药的患病细胞（颜色：如耐药性癌细胞）。