公开(公告)号：EP1465489A4

公开(公告)日：2005-09-28

申请号：EP02780657

申请日：2002-11-14

申请人： MEDTRONIC SOFAMOR DANEK

发明人： MCKAY WILLIAM F ,  BODEN SCOTT D ,  YOON SANGWOOK T

IPC分类号： A61K35/12 ,  A61K48/00 ,  A61L27/38 ,  C07K14/47 ,  C07K14/78 ,  C07K16/18 ,  C12N5/08 ,  C12N5/22 ,  C12N5/16 ,  C12N15/63

CPC分类号： A61K48/005 ,  A61K35/12 ,  A61K35/13 ,  A61K48/00 ,  A61K2121/00 ,  A61L27/3804 ,  A61L27/3856 ,  C07K14/47 ,  C07K14/4702 ,  C07K14/78 ,  C07K16/18 ,  C12N2799/022

摘要： Methods of expressing LIM mineralization protein in non-osseous mammalian cells, such as stem cells or intervertebral disc cells (e.g., cells of the annulus fibrosus, or cells of the nucleus pulposus) are described. The methods involve transfecting the cells with an isolated nucleic acid comprising a nucleotide sequence encoding a LIM mineralization protein operably linked to a promoter. Transfection may be accomplished ex vivo or in vivo by direct injection of virus or naked DNA, or by a nonviral vector such as a plasmid. Expression of the LIM mineralization protein can stimulate proteoglycan and/or collagen production in cells capable of producing proteoglycyan and/or collagen. Methods for treating disc disease associated with trauma or disc degeneration are also described.