公开(公告)号：EP3113760A4

公开(公告)日：2017-08-23

申请号：EP15759285

申请日：2015-03-04

申请人： MEMORIAL SLOAN KETTERING CANCER CENTER

发明人： RENSTROM ERIK ,  STETTER ERNST ,  KIRCHER MORITZ F ,  KOCH MARTIN

IPC分类号： A61K9/14 ,  A61N2/06 ,  B82Y30/00 ,  C07K16/30

CPC分类号： C07K16/2896 ,  A61K9/0009 ,  A61K41/0028 ,  A61K47/6849 ,  B82Y5/00 ,  B82Y30/00 ,  C07K2317/73 ,  C07K2317/77

摘要： Dynamic magnetic field (DMF) treatment to direct movement, and specifically rotation, of magnetic particles associated with a target structure, so that mechanical force is applied to the target structure. DMF treatment directs rotation about the axis in individual magnetic particles (e.g., superparamagnetic nanoparticles ), effecting permeabilization and/or other disruption of membranes (e.g., cell membranes and/or intracellular membranes). Cell death is remotely induced by application of mechanical force through rotational movement of magnetic particles (specifically superparamagnetic iron oxide nanoparticles, "SPIONs") exposed to DMF treatment. Particles were specifically targeted to lysosomal membrane structures, and were individually induced through application of DMF treatment to rotate about their axes. The resulting mechanical force disrupted (e.g., permeabilized) the lysosomal membranes, releasing lysosomal enzymes and triggering apoptosis, all without heating the particles (or their surroundings).

摘要翻译： 动态磁场（DMF）处理以引导与目标结构相关联的磁性粒子的移动，特别是旋转，从而机械力施加到目标结构。 DMF处理引导围绕各个磁性颗粒（例如，超顺磁性纳米颗粒）中的轴的旋转，影响膜（例如细胞膜和/或细胞内膜）的透化和/或其他破裂。 通过施加机械力通过暴露于DMF处理的磁性颗粒（特别是超顺磁性氧化铁纳米颗粒，“SPION”）的旋转运动远程诱导细胞死亡。 颗粒特异性地靶向溶酶体膜结构，并且通过应用DMF处理以围绕它们的轴旋转而单独诱导。 所产生的机械力破坏（例如透化）溶酶体膜，释放溶酶体酶并触发凋亡，所有这些都不加热颗粒（或其周围环境）。