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1.发明公开Specific binding assay based on enzyme cascade amplification 失效Spezifischer Bindungstest auf der Basis von Enzym-Kaskade-Vergrösserung。
公开(公告)号：EP0144744A2
公开(公告)日：1985-06-19
申请号：EP84113237.6
申请日：1984-11-03
IPC分类号： G01N33/53 , G01N33/537 , G01N33/542
CPC分类号： G01N33/542 , G01N33/535 , G01N33/581
摘要： The present invention provides specific binding assay methods and reagent systems for the quantitative or qualitative determination of an analyte in a liquid medium based on heterogeneous or homogeneous protocols. In particular, the invention relates to the use of specific binding assay techniques wherein the label is a participant in or a modulator of an enzymatic reaction whereby a first enzyme acts on a first substrate to produce a second enzyme. The second enzyme can be used to catalyze a detectable response or can act on a second substrate to produce a third enzyme. The label is chosen from the first substrate or a cofactor or modulator of the first enzyme. In one embodiment, the first substrate is a zymogen which when acted upon by the first enzyme produces a second enzyme. When the final enzyme product is used to catalyze the production of a detectable response proportional of the concentration of the analyte, amplification results providing a very sensitive assay.
摘要翻译： 本发明提供了用于基于异质或均质方案定量或定性地测定液体介质中的分析物的特异性结合测定方法和试剂系统。 特别地，本发明涉及特异性结合测定技术的用途，其中标记是酶反应的参与者或调节剂，由此第一酶作用于第一底物以产生第二酶。 第二种酶可用于催化可检测的反应或可以作用于第二底物以产生第三种酶。 标签选自第一底物或第一酶的辅因子或调节剂。 在一个实施方案中，第一底物是酶原，当被第一种酶作用时产生第二种酶。 当最终的酶产物用于催化与分析物浓度成比例的可检测反应的产生时，扩增结果提供非常敏感的测定。
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公开(公告)号：EP0193061A3
公开(公告)日：1987-08-12
申请号：EP86101968
申请日：1986-02-17
CPC分类号： G01N33/521 , C12Q1/54 , Y10T436/104998
摘要： A viability test device wherein the viability of a test composition for the determination of an analyte of interest can be determined by simply wetting the device with ordinary water. The viability device can be prepared in three formats: a control or calibrator device, an internal control device and a self indicating device. Unreacted analyte, or an analog thereof, can be incorporated in a limited defined portion of a dried carrier matrix incorporated with a test composition to provide an internal control test device. When wetted with water, a positive optical response, usually color, indicates the test composition can provide a viable test for the analyte. The internal control test device is particularly advantageously used by diabetics in their own homes where a negative test response could be due to the desired control of the user's condition or due to deterioration of a test composition because of unfavourable storage conditions.
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