公开(公告)号：EP1185616A4

公开(公告)日：2003-04-23

申请号：EP00930821

申请日：2000-05-19

申请人： MILLIPORE CORP

发明人： PRESENTE ESTHER ,  YOUNG BARBARA ,  UPPERMAN SUSAN

IPC分类号： G01N33/483 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/22 ,  C12Q1/42 ,  C12Q1/48 ,  C12Q1/66 ,  G01N21/78 ,  C12N1/06 ,  C12N13/00 ,  C12Q1/06 ,  C12Q1/34

CPC分类号： C12Q1/48 ,  C12Q1/04 ,  C12Q1/22 ,  C12Q1/66 ,  C12Q2304/60

摘要： A method for rapidly detecting and enumerating microorganisms, having a level of microbial ATP, in the presence of mammalian cell preparations, comprising the steps of: providing a mammalian cell preparation comprising mammalian cells having a level of mammalian ATP; reducing the level of mammalian ATP in the cell preparation by, differentially lyzing the mammalian cells with detergent or osmotic shock to extract the mammalian ATP, treating the extracted mammalian ATP with one or more ATP hydrolyzing compounds; and immobilizing the microorganisms and washing away the detergent and the hydrolyzing enzyme by filtering the mammalian cell preparation through a micropartitioned hydrophilic/hydrophobic membrane; extracting the microbial ATP using an extracting reagent; drying the membrane; applying a bioluminescent reagent onto the membrane; and detecting and enumerating the microorganisms.