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公开(公告)号：EP0403679A1
公开(公告)日：1990-12-27
申请号：EP89111405.0
申请日：1989-06-22
CPC分类号： B01D63/08 , B01D61/18 , B01L3/50255
摘要： A multi-well filtration apparatus for the assay of microliter quantities is provided wherein a filter (30) is positioned in each well (15) on a plate (13) having an open spout (34) positioned in a the plate. The spout (34) has a collar (37) on its outer surface extending in a direction perpendicular to the vertical axis of the spout. The collar prevents a liquid droplet from climbing the outer surface of the spout from its open end.
摘要翻译： 提供了用于测定微升量的多孔过滤装置，其中过滤器（30）位于板（13）上的每个孔（15）中，板（13）具有位于板中的开口喷口（34）。 喷口（34）在其外表面上具有沿垂直于喷口的竖直轴线的方向延伸的套环（37）。 套环防止液滴从其开口端爬出喷嘴的外表面。
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公开(公告)号：EP0295566A1
公开(公告)日：1988-12-21
申请号：EP88109143.3
申请日：1988-06-08
CPC分类号： G01N1/4077 , B01D61/18 , B01D63/087 , B01D65/08 , B01D2321/2083 , B01L3/50273 , B01L3/502753 , G01N2001/4088
摘要： A filter system particularly useful with small sample volumes includes first and second housing sections with a flow channel extending therebetween. A membrane filter forms one boundary of the flow channel. A pair of reservoirs, one for feed and the other for permeate collection, are integrally formed with the first housing section. A fluid communication path is established from the first section to the second section and then through means of a deformable chamber to the flow channel. The deformable chamber is adjacent to a rigid surface that is integral with one of the housing sections and in this manner is adapted to pump fluid through the system when interfacing with a pump.
摘要翻译： 特别适用于小样品体积的过滤系统包括第一和第二壳体部分，其中在其间延伸有流动通道。 膜过滤器形成流动通道的一个边界。 一对储存器，一个用于进料，另一个用于渗透物收集，与第一壳体部分整体形成。 从第一部分到第二部分建立流体连通路径，然后通过可变形室到流动通道的装置。 可变形室与刚性表面相邻，该刚性表面与一个壳体部分成一体，并且以这种方式适于在与泵接口时将流体泵送通过系统。
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公开(公告)号：EP0815915A1
公开(公告)日：1998-01-07
申请号：EP97110162.1
申请日：1997-06-20
CPC分类号： F16K7/20 , B01D36/001 , F16K24/04
摘要： Disclosed is a vacuum control valve for manually controlling pressure in a vacuum device, such as a vacuum filtration device. In one embodiment, the vacuum control valve comprises (a) an inlet adapted to receive a vacuum port, (b) an outlet in fluid communication with the inlet and adapted to receive a vacuum source; (c) a vacuum vent providing fluid communication among the inlet, the outlet and a gas source; and (d) a vent cap movably mounted to the vacuum vent and capable of sealing the vacuum vent from the gas source. The vent cap can be formed of a flexible, for example, elastomeric, material and, when manually deformed, is displaced from the vacuum vent, thereby opening the vacuum vent. The vent cap can include a tab for actuating the valve such that applying a force to the tab deforms the vent cap, displacing it from the vacuum vent and opening the vacuum vent to the gas source.
The invention also provides a vacuum filtration device comprising (a) a filter body including a filter membrane; (b) a passageway extending through the body and communicating with the upstream side of the filter for receiving a fluid to be filtered; (c) a junction mounted on the body on the downstream side of the filter and adapted to receive a closed container for receiving the filtered fluid and including sealing means for creating a fluid tight seal between the container and the body; (d) a vacuum port communicating with said junction and adapted to be connected to a vacuum source for drawing the fluid through the filter and comprising a sealable vacuum vent which, when open, provides fluid communication between the vacuum port and a gas source.摘要翻译： 公开了一种用于手动控制真空装置（例如真空过滤装置）中的压力的真空控制阀。 在一个实施例中，真空控制阀包括（a）适于接收真空端口的入口，（b）与入口流体连通并适于接收真空源的出口; （c）提供入口，出口和气体源之间的流体连通的真空排气口; 和（d）可移动地安装到真空排气口并且能够从气体源密封真空排气口的通气盖。 通气盖可以由柔性例如弹性体材料形成，并且当手动变形时，其从真空排气口移位，从而打开真空排气口。 通气盖可以包括用于致动阀的突片，使得向突片施加力使通气盖变形，使其从真空排气口移出并将真空排气口打开到气体源。 本发明还提供一种真空过滤装置，其包括（a）包括过滤膜的过滤体; （b）延伸穿过本体并与过滤器的上游侧连通以用于接收待过滤流体的通道; （c）在过滤器的下游侧安装在主体上并且适于容纳用于接收过滤流体的密封容器并且包括用于在容器和主体之间产生流体密封的密封装置的接头; （d）与所述接合部连通并且适于连接到真空源的真空端口，用于将流体抽吸通过过滤器并且包括可密封的真空通气口，其在打开时提供真空端口和气体源之间的流体连通。
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公开(公告)号：EP0332944A2
公开(公告)日：1989-09-20
申请号：EP89103676.6
申请日：1989-03-02
CPC分类号： B01L99/00 , B01D29/01 , B01D29/50 , B01D29/96 , B01D2201/24 , G01N1/40 , G01N2001/2873 , G01N2001/288
摘要： A filter punch construction is provided for use in conjunction with a multiwell filtration device wherein each well includes a filtration membrane. The punch has a leading flat surface to which is secured an elongated piercing member having a sharp point and a following surface to which is secured a guide member. The piercing member pierces the filtration membrane in each well and the flat leading surface pushes the entire membrane and retentate thereon into a receptacle vial.
摘要翻译： 提供了与多孔过滤装置结合使用的过滤冲孔结构，其中每个井都包括一个过滤膜。 冲头具有前导平面，固定有具有尖锐点的细长刺穿构件和紧固引导构件的后续表面。 刺穿构件穿透每个孔中的过滤膜，平坦的前表面推动整个膜并在其上滞留在容器小瓶中。
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5.发明授权
公开(公告)号：EP1398082B1
公开(公告)日：2012-07-11
申请号：EP03253136.0
申请日：2003-05-20
IPC分类号： B01L3/00
CPC分类号： B01L3/50255 , B01J20/28026 , B01J20/28033 , B01L2200/0631 , B01L2300/0681 , B01L2300/0829 , B01L2400/049 , G01N1/40 , G01N1/405 , G01N27/44717 , Y10T436/10 , Y10T436/25 , Y10T436/25125 , Y10T436/25375
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公开(公告)号：EP0332944A3
公开(公告)日：1990-04-11
申请号：EP89103676.6
申请日：1989-03-02
CPC分类号： B01L99/00 , B01D29/01 , B01D29/50 , B01D29/96 , B01D2201/24 , G01N1/40 , G01N2001/2873 , G01N2001/288
摘要： A filter punch construction is provided for use in conjunction with a multiwell filtration device wherein each well includes a filtration membrane. The punch has a leading flat surface to which is secured an elongated piercing member having a sharp point and a following surface to which is secured a guide member. The piercing member pierces the filtration membrane in each well and the flat leading surface pushes the entire membrane and retentate thereon into a receptacle vial.
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7.发明公开
公开(公告)号：EP1398082A1
公开(公告)日：2004-03-17
申请号：EP03253136.0
申请日：2003-05-20
IPC分类号： B01L3/00
CPC分类号： B01L3/50255 , B01J20/28026 , B01J20/28033 , B01L2200/0631 , B01L2300/0681 , B01L2300/0829 , B01L2400/049 , G01N1/40 , G01N1/405 , G01N27/44717 , Y10T436/10 , Y10T436/25 , Y10T436/25125 , Y10T436/25375
摘要： An integrated proteomics sample preparation device and method for in-gel digestion of proteins and for desalting and concentrating samples prior to further analysis such as by MALDI TOF and/or electro-spray ionization (ESI) mass spectrometry. The device and method of the present invention allow for digestion, desalting and concentration of sample prior to analysis. More specifically, the device in accordance with an embodiment of the present invention includes a plurality of wells in fluid communication with a an outlet or drainage opening containing a three dimensional structure comprising a plurality of sorptive particles entrapped in a porous polymer matrix so as to form a device capable of carrying out solid phase extraction. In a preferred embodiment, the wells are configured so as to prevent a sample carrier present in the wells from clogging the outlet when subjected to a driving force such as vacuum. The device also reduces or eliminates overflowing of a well in the event a drain becomes clogged during automated operation.
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公开(公告)号：EP0815915B1
公开(公告)日：2003-03-19
申请号：EP97110162.1
申请日：1997-06-20
IPC分类号： F16K7/00
CPC分类号： F16K7/20 , B01D36/001 , F16K24/04
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公开(公告)号：EP0403679B1
公开(公告)日：1994-01-05
申请号：EP89111405.0
申请日：1989-06-22
CPC分类号： B01D63/08 , B01D61/18 , B01L3/50255
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10.发明公开
公开(公告)号：EP0393478A2
公开(公告)日：1990-10-24
申请号：EP90106843.7
申请日：1990-04-10
发明人： Rising, Donald B. , Zimmerman, Rita
IPC分类号： B01D57/02 , G01N27/447
CPC分类号： B01D57/02 , G01N27/44704 , G01N27/44773
摘要： An apparatus and process are provided for transferring a gel shaped as a cylinder (18) containing a treated sample from a first step to a second electrophoresis step without distorting the position of the treated sample within the shaped gel. A shaped gel useful in electrophoresis is formed in a tube with a multifilament thread (14) extending the entire length of and within the central portion of the shaped gel. The shaped gel then is employed in a first treatment step to form a treated sample that may be a separated sample. After extrusion of the gel from the tube, the portion of the thread extending from each end (26, 28) of the shaped gel is grasped and the shaped gel containing the treated sample is transferred to contact a slab gel (30) along the entire length of the shaped gel to effect an electrophoresis step in the second treatment step.
摘要翻译： 提供了一种装置和方法，用于将含有经处理的样品的圆筒（18）的凝胶从第一步骤转移到第二电泳步骤，而不会使经处理的样品在成形凝胶内的位置变形。 在具有在成形凝胶的中心部分的整个长度和延伸的复丝螺纹（14）的管中形成可用于电泳的成形凝胶。 然后将成形的凝胶用于第一处理步骤以形成可以是分离的样品的经处理的样品。 在从管中挤出凝胶后，抓住从成形凝胶的每个端部（26,28）延伸的部分螺纹，并将含有处理过的样品的成形凝胶转移成沿整个接触板坯凝胶（30） 成型凝胶的长度以在第二处理步骤中进行电泳步骤。
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