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公开(公告)号：EP2174714B1
公开(公告)日：2013-04-17
申请号：EP10152440.3
申请日：2003-10-20
CPC分类号： B01D67/0009 , B01D2323/24 , B01D2325/12 , B01L3/50255 , B01L2200/12 , B01L2300/0829 , B01L2300/0851 , B01L2400/049 , Y10T436/25375 , Y10T436/255
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公开(公告)号：EP1783494A1
公开(公告)日：2007-05-09
申请号：EP06255512.3
申请日：2006-10-26
IPC分类号： G01N33/543 , B01L3/00
CPC分类号： G01N33/5302 , B01L3/50255 , B01L2400/0487 , G01N33/54366 , G01N33/54393 , G01N33/561 , Y10T436/2575
摘要： A rapid, efficient and convenient method to detect one or more biological entities on a blotting membrane is provided. The detection can relate to the position, nature or amount of the biological substance on one or more membranes. The invention method involves a pressure assisted regiment (such as vacuum or positive gas pressure) for the supply and removal of reagents and permits washing of the contaminants from substances embedded in the membrane to be detected using very low volumes of liquid. This method enables completion of the blocking, washing and antibody binding steps in about 30 minutes without comprising blot quality. In another aspect, the invention is directed to an apparatus useful in conducting the method of the invention.
The device is comprised of several layers including a porous support layer below the blotting membrane(s), a flow distributor above the blotting membrane(s) and a well on the flow distributor to contain the liquid to the desired area and to allow for lower starting volumes of such liquid. Preferably, the flow distributor is a non-binding or low binding hydrophilic porous membrane such as a 0.22 micron membrane.摘要翻译： 提供了一种快速，有效和方便地检测印迹膜上的一个或多个生物实体的方法。 检测可以涉及一个或多个膜上生物物质的位置，性质或数量。 本发明方法包括用于供应和去除试剂的压力辅助团（例如真空或正气压），并且允许使用非常低体积的液体从嵌入待检测的膜的物质中洗涤污染物。 该方法能够在约30分钟内完成封闭，洗涤和抗体结合步骤，而不包括印迹质量。 在另一方面，本发明涉及可用于实施本发明的方法的装置。 该装置包括多个层，包括印迹膜下方的多孔支撑层，印迹膜上方的流动分布器和流量分配器上的孔，以将液体包含在期望的区域并允许较低的 开始这种液体的体积。 优选地，流动分配器是非结合或低结合的亲水性多孔膜，例如0.22微米的膜。
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3.发明公开Apparatus and method for sample preparation and direct spotting of eluants onto a maldi-tof target 有权装置和方法用于样品制备和洗脱液的直接点样上的MALDI-TOF目标
公开(公告)号：EP1398614A2
公开(公告)日：2004-03-17
申请号：EP03017402.3
申请日：2003-07-31
CPC分类号： G01N1/405 , B01L3/0262 , B01L3/50255 , B01L2200/0631 , B01L2300/0829 , H01J49/0418 , H01J49/40 , Y10T436/255 , Y10T436/2575
摘要： A single- or multi-well sample preparation apparatus and method for desalting, concentrating and depositing samples prior to further analysis such as by MALDI TOF mass spectrometry. The apparatus in accordance with an embodiment of the present invention includes a plurality of wells each in fluid communication with a respective outlet or drainage opening (15), optionally containing a three dimensional membrane structure (25) preferably comprising a plurality of sorptive particles entrapped in a porous polymer matrix so as to form a device capable of carrying out solid phase extraction. The apparatus is designed to allow for direct spotting onto a MALDI target, thereby eliminating a transfer step. Also disclosed is a method of sample preparation, deposition and analysis using the apparatus of the present invention.
摘要翻译： 单层或多层孔样品制备装置和方法，用于脱盐，浓缩和沉积样品之前つweiterer分析：如通过MALDI TOF质谱法。 与在本发明的实施方式中雅舞蹈该装置包括：各自与respectivement出口或排水开口（15）流体连通的孔的复数，任选地含有一个三维膜结构（25）优选地包括在夹带的吸着性颗粒的多元 多孔聚合物基质，从而形成能够进行固相提取的装置。 该装置被设计成允许直接点样到MALDI目标，从而消除了转印步骤。 所以圆盘游离缺失是使用本发明的装置的样品制备，沉积和分析的方法。
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公开(公告)号：EP0458198B1
公开(公告)日：1996-07-24
申请号：EP91107981.2
申请日：1991-05-16
发明人： Clark, Phillip
IPC分类号： B01L3/00 , G01N33/543 , G01N33/53
CPC分类号： G01N33/54386 , G01N33/54366 , Y10S436/807 , Y10T436/25375
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公开(公告)号：EP0458198A3
公开(公告)日：1992-11-19
申请号：EP91107981.2
申请日：1991-05-16
发明人： Clark, Phillip
IPC分类号： B01L3/00 , G01N33/543 , G01N33/53
CPC分类号： G01N33/54386 , G01N33/54366 , Y10S436/807 , Y10T436/25375
摘要： Assay apparatus (10) is provided which includes a filter (24) such as a porous membrane, a volume (18) that can contain an absorbent and flexible means (20) for reducing the volume (18) in order to effect passage of liquid sample through the membrane (24) and into the volume (18). The assay apparatus can be produced by a continuous manufacturing process.
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公开(公告)号：EP3628733A1
公开(公告)日：2020-04-01
申请号：EP19210321.6
申请日：2012-07-06
发明人： Scott, Christopher A. , Greenizen, Kurt , Gutierrez, Sara D. , Briggs, David , Scaduto, Ralph T. , Nadler, Timothy K. , Bonhomme, Luis , Mabuchi, Masaharu , Clark, Phillip
摘要： A sample preparation device comprises a reservoir/exchange member (12) including a sample reservoir (14) and a column in fluid communication with the sample reservoir (14). The column has a holder region (20) and a region (22) having a finned geometry tapering radially from a relatively thick upper portion (22a) to a relatively thin lower portion (22b) that converges in an axially extending stem (23) having an open bottom. A filter (50) is or is capable of being sealingly engaged to the holder region (20). The filter (50) includes spaced membranes. The region (22) having a finned geometry positions between the spaced membranes.
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公开(公告)号：EP3056276B1
公开(公告)日：2018-09-26
申请号：EP16155180.9
申请日：2016-02-11
发明人： Clark, Phillip , Greenizen, Kurt , Doyle, John
IPC分类号： G01N1/40
CPC分类号： G01N1/4005 , B01L3/502 , B01L2200/026 , B01L2200/0689 , B01L2200/085 , B01L2200/087 , B01L2300/042 , B01L2300/048 , B01L2300/049 , B01L2300/0681 , B01L2300/14 , B01L2400/0487 , B01L2400/0605 , B01L2400/0633 , B01L2400/065
摘要： Fluid containment pressure vessel that eliminates the outer support housing present in certain conventional vessels, and provides an improved cap-to-body interface. In certain embodiments, the cap is threaded and configured to threadingly engage with the body of the pressure vessel, and has sufficient structural integrity to withstand the pressures in the device without the need for a support housing. In certain embodiments, an interlock mechanism is provided that prevents the cap from being opened (e.g., removed from the body) while the device is under pressure. In certain embodiments, a pressure relief valve is provided with a pre-loaded biasing mechanism to achieve the required pressure release rate. The vessel can be used for sample preparation, including purification or concentration of samples, particularly protein samples.
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公开(公告)号：EP3056276A3
公开(公告)日：2016-11-16
申请号：EP16155180.9
申请日：2016-02-11
发明人： Clark, Phillip , Greenizen, Kurt , Doyle, John
CPC分类号： G01N1/4005 , B01L3/502 , B01L2200/026 , B01L2200/0689 , B01L2200/085 , B01L2200/087 , B01L2300/042 , B01L2300/048 , B01L2300/049 , B01L2300/0681 , B01L2300/14 , B01L2400/0487 , B01L2400/0605 , B01L2400/0633 , B01L2400/065
摘要： Fluid containment pressure vessel that eliminates the outer support housing present in certain conventional vessels, and provides an improved cap-to-body interface. In certain embodiments, the cap is threaded and configured to threadingly engage with the body of the pressure vessel, and has sufficient structural integrity to withstand the pressures in the device without the need for a support housing. In certain embodiments, an interlock mechanism is provided that prevents the cap from being opened (e.g., removed from the body) while the device is under pressure. In certain embodiments, a pressure relief valve is provided with a pre-loaded biasing mechanism to achieve the required pressure release rate. The vessel can be used for sample preparation, including purification or concentration of samples, particularly protein samples.
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公开(公告)号：EP1074292B1
公开(公告)日：2004-07-28
申请号：EP00121976.5
申请日：1996-05-16
发明人： Clark, Phillip , Foley, Brian D. , Pitt, Aldo M.
CPC分类号： B01D63/087 , B01D29/012 , B01D29/05 , B01D36/001 , B01D61/18 , B01D61/20 , B01D63/081 , B01D2201/204 , B01L3/502 , B01L3/50255 , B29C65/08 , B29C65/561 , B29C66/1142 , B29C66/54 , B29C66/5412 , B29C66/543 , B29C66/71 , B29C66/727 , B29C66/7392 , B29C66/73921 , B29K2001/12 , B29L2031/14 , B29K2077/00 , B29K2023/10 , B29K2069/00 , B29K2027/18 , B29K2023/06 , B29K2081/06 , B29K2027/16 , B29K2023/12 , B29K2001/00
摘要： A vacuum filter device is disclosed which includes a filter body (11) which is adapted to receive in fluid-tight, sealed relationship a pair of closed containers (15,16) for solutions to be filtered by means of a membrane filter positioned within the filter body. A vacuum port (23) in the filter body (11) communicates with the downstream side of the membrane and a vent passageway (60;70) also located in the filter body (11) communicates with the closed sample container to serve as a vent to atmospheric pressure. The vent passageway is sealed by an air permeable hydrophobic filter to prevent the sample solutions from leaking out of the device during normal use.
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10.发明公开Apparatus and method for sample preparation and direct spotting of eluants onto a maldi-tof target 有权装置和方法用于样品制备和洗脱液的直接点样上的MALDI-TOF目标
公开(公告)号：EP1398614A3
公开(公告)日：2004-06-23
申请号：EP03017402.3
申请日：2003-07-31
CPC分类号： G01N1/405 , B01L3/0262 , B01L3/50255 , B01L2200/0631 , B01L2300/0829 , H01J49/0418 , H01J49/40 , Y10T436/255 , Y10T436/2575
摘要： A single- or multi-well sample preparation apparatus and method for desalting, concentrating and depositing samples prior to further analysis such as by MALDI TOF mass spectrometry. The apparatus in accordance with an embodiment of the present invention includes a plurality of wells each in fluid communication with a respective outlet or drainage opening (15), optionally containing a three dimensional membrane structure (25) preferably comprising a plurality of sorptive particles entrapped in a porous polymer matrix so as to form a device capable of carrying out solid phase extraction. The apparatus is designed to allow for direct spotting onto a MALDI target, thereby eliminating a transfer step. Also disclosed is a method of sample preparation, deposition and analysis using the apparatus of the present invention.
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