公开(公告)号：EP3108218A2

公开(公告)日：2016-12-28

申请号：EP15751396.1

申请日：2015-02-20

申请人： Massachusetts Institute Of Technology

发明人： CHEN, Fei ,  TILLBERG, Paul, Warren ,  BOYDEN, Edward, Stuart

IPC分类号： G01N1/00 ,  G01N1/28 ,  G01N1/30 ,  G01N1/36

CPC分类号： G01N1/36 ,  C12Q1/6841 ,  G01N1/30 ,  G01N2001/364 ,  G02B21/0072

摘要： The present invention relates to an enlarged sample of interest for microscopy and methods for enlarging a sample of interest and the optical imaging of a sample of interest with resolution better than the classical microscopy diffraction limit, by synthesizing a swellable polymer network within a sample, it can be physically expanded, resulting in physical magnification.

公开(公告)号：EP3332258B1

公开(公告)日：2020-01-01

申请号：EP16751783.8

申请日：2016-08-05

申请人： Massachusetts Institute of Technology

发明人： TILLBERG, Paul, Warren ,  CHEN, Fei ,  BOYDEN, Edward Stuart ,  YU, Chih-Chieh

IPC分类号： G01N33/68 ,  G01N1/36

公开(公告)号：EP3332258A1

公开(公告)日：2018-06-13

申请号：EP16751783.8

申请日：2016-08-05

申请人： Massachusetts Institute of Technology

发明人： TILLBERG, Paul, Warren ,  CHEN, Fei ,  BOYDEN, Edward Stuart ,  YU, Chih-Chieh

IPC分类号： G01N33/68 ,  G01N1/36

摘要： The invention provides a method termed protein retention ExM (proExM), in which proteins, rather than labels, are anchored to the swellable gel, using a cross-linking molecule. This proExM strategy can be used to perform nanoscale imaging of immunostained cells and tissues as well as samples expressing various FPs as fluorescent signals from genetically encoded fluorescent proteins and/or conventional fluorescently labeled secondary antibodies and streptavidin that are directly anchored to the gel are preserved even when subjected to the nonspecific proteolytic digestion.