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    Method of increasing replication of a reassortant influenza virus in eggs.
    1.
    发明公开
    Method of increasing replication of a reassortant influenza virus in eggs. 有权
    方法um die Replikation eines rekombinanten Grippenvirus in Eiern zu erhhenhen

    公开(公告)号：EP2644696A1

    公开(公告)日：2013-10-02

    申请号：EP13159978.9

    申请日：2005-05-20

    申请人： MedImmune, LLC

    发明人： Hoffmann, Erich ,  Jin, Hong ,  Lu, Bin ,  Duke, Gregory ,  Kemble, George ,  Chen, Zhongying

    IPC分类号： C12N7/02 ,  A61K39/145 ,  C12P19/34

    CPC分类号： C07K14/005 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/5254 ,  C12N7/00 ,  C12N2760/16134 ,  C12N2760/16221 ,  C12N2760/16222 ,  C12N2760/16234 ,  C12N2760/16243 ,  C12N2760/16261

    摘要： The present invention relates to a method of increasing replication of a reassortant influenza virus by at least 10%, the method comprising the steps of: i) comparing the amino acid sequence of the reassortant influenza virus with the amino acid sequence of a different influenza virus, which different influenza virus grows in embryonated eggs; and ii) substituting as needed HA H3 amino acid residues such that position 186 is a valine and position 226 is an isoleucine. The present invention further relates to a replication enhanced reassortant influenza virus comprising an HA H3 protein comprising a valine at position 186 and an isoleucine at position 226. Furthermore, the invention relates to an immunogenic composition comprising the replication enhanced reassortant influenza virus comprising an HA H3 protein comprising a valine at position 186 and an isoleucine at position 226.

    摘要翻译： 本发明涉及将重配流感病毒的复制增加至少10％的方法，所述方法包括以下步骤：i）将重配流感病毒的氨基酸序列与不同流感病毒的氨基酸序列进行比较 ，不同流感病毒在胚胎卵中生长; 和ii）根据需要替换HA H3氨基酸残基，使得位置186是缬氨酸，位置226是异亮氨酸。 本发明还涉及复制增强重配流感病毒，其包含HA H3蛋白，其包含位置186处的缬氨酸和226位的异亮氨酸。此外，本发明涉及包含复制增强重配流感病毒的免疫原性组合物，其包含HA H3 蛋白质包含位于第186位的缬氨酸和226位的异亮氨酸。