公开(公告)号：EP2955226A1

公开(公告)日：2015-12-16

申请号：EP14748891.0

申请日：2014-02-07

申请人： Medical & Biological Laboratories Co., Ltd.

发明人： ONO, Kenichiro ,  YAGI, Kasumi

IPC分类号： C12N15/09 ,  A61K39/395 ,  A61P35/00 ,  C07K16/18 ,  C12N5/10 ,  C12P21/08

CPC分类号： C07K16/22 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/33 ,  C07K2317/34 ,  C07K2317/73 ,  C07K2317/76 ,  C12N15/09 ,  C12N15/85

摘要： An object is to provide an antibody capable of specifically recognizing a human NRG1 protein isoform, and suppressing signal transduction in which the isoform is involved. An antibody capable of binding to a region at positions 221 to 234 of a human NRG1-α protein or an antibody capable of binding to a region at positions 213 to 239 of a human NRG1-β1 protein was successfully obtained. Further, it was also found that these antibodies had an activity of suppressing cleavage of the NRG1 protein, an activityofsuppressing phosphorylation of an ErbB3 protein in a cancer cell, and an activity of suppressing in vivo tumor proliferation.

摘要翻译： 目的是提供能够特异性识别人类NRG1蛋白质同种型的抗体，抑制其中涉及同种型的信号转导。 成功获得能够结合人类NRG1-±蛋白质221〜234位的区域的抗体或能结合人类NRG1-2蛋白质213〜239位的抗体的抗体。 此外，还发现这些抗体具有抑制NRG1蛋白的切割，抑制癌细胞中ErbB3蛋白的磷酸化的活性和抑制体内肿瘤增殖的活性的活性。

公开(公告)号：EP2881467B1

公开(公告)日：2018-10-31

申请号：EP13825402.4

申请日：2013-07-30

申请人： National University Corporation Nagoya University ,  Medical & Biological Laboratories Co., Ltd.

发明人： KADOMATSU, Kenji ,  KISHIDA, Satoshi ,  ONO, Kenichiro ,  YAGI, Kasumi

IPC分类号： C12N15/09 ,  A61K39/395 ,  A61P35/00 ,  C07K16/18 ,  C07K16/46 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12P21/08 ,  C07K16/22

CPC分类号： C07K16/22 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/565 ,  C07K2317/73 ,  C07K2317/76 ,  C07K2317/90

摘要： A monoclonal antibody, which recognizes at least two amino acids among amino acids located at position 69, position 79, position 81 and position 102 of human midkine, has been found to have excellent reactivity with and excellent neutralizing activity against human midkine. Moreover, the activity of suppressing the proliferation of tumor has been observed in the antibody having excellent neutralizing activity. The use of the antibody of the present invention makes it possible to treat cancer effectively and to detect or purify midkine efficiently.

公开(公告)号：EP2955226B1

公开(公告)日：2019-06-26

申请号：EP14748891.0

申请日：2014-02-07

申请人： Medical & Biological Laboratories Co., Ltd.

发明人： ONO, Kenichiro ,  YAGI, Kasumi

IPC分类号： C12N15/09 ,  A61K39/395 ,  A61P35/00 ,  C07K16/18 ,  C12N5/10 ,  C12P21/08

公开(公告)号：EP2881467A1

公开(公告)日：2015-06-10

申请号：EP13825402.4

申请日：2013-07-30

申请人： National University Corporation Nagoya University ,  Medical & Biological Laboratories Co., Ltd.

发明人： KADOMATSU, Kenji ,  KISHIDA, Satoshi ,  ONO, Kenichiro ,  YAGI, Kasumi

IPC分类号： C12N15/09 ,  A61K39/395 ,  A61P35/00 ,  C07K16/18 ,  C07K16/46 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12P21/08

CPC分类号： C07K16/22 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/565 ,  C07K2317/73 ,  C07K2317/76 ,  C07K2317/90

摘要： A monoclonal antibody, which recognizes at least two amino acids among amino acids located at position 69, position 79, position 81 and position 102 of human midkine, has been found to have excellent reactivity with and excellent neutralizing activity against human midkine. Moreover, the activity of suppressing the proliferation of tumor has been observed in the antibody having excellent neutralizing activity. The use of the antibody of the present invention makes it possible to treat cancer effectively and to detect or purify midkine efficiently.

摘要翻译： 已经发现，在人类midkine的位置69，位置79，位置81和位置102的氨基酸中识别至少两个氨基酸的单克隆抗体具有优异的与人类midkine的反应性和优异的中和活性。 此外，在具有优异的中和活性的抗体中观察到抑制肿瘤增殖的活性。 使用本发明的抗体可以有效地治疗癌症并有效地检测或纯化中期因子。