公开(公告)号：EP0337589B1

公开(公告)日：1993-06-23

申请号：EP89300328.5

申请日：1989-01-13

申请人： Merck & Co., Inc.

发明人： Liberator, Paul A. ,  Nollstadt, Karl H. ,  Turner, Mervyn J. ,  St. John Crane, Mark ,  Karkhanis, Yashwant D. ,  Chakraborty, Prasanta R. ,  Profous-Juchelka

IPC分类号： C12N15/30 ,  C12P21/02 ,  A61K39/012 ,  C07K13/00

CPC分类号： C07K14/455 ,  A61K39/00 ,  C07K16/20

公开(公告)号：EP0337589A3

公开(公告)日：1989-10-25

申请号：EP89300328.5

申请日：1989-01-13

申请人： Merck & Co., Inc.

发明人： Liberator, Paul A. ,  Nollstadt, Karl H. ,  Turner, Mervyn J. ,  St. John Crane, Mark ,  Karkhanis, Yashwant D. ,  Chakraborty, Prasanta R. ,  Profous-Juchelka

IPC分类号： C12N15/30 ,  C12P21/02 ,  A61K39/012 ,  C07K13/00

CPC分类号： C07K14/455 ,  A61K39/00 ,  C07K16/20

摘要： Genes coding for novel Group B Eimeria tenella protein immunogens have been isolated and inserted into a novel expression vector which in turn has been used to transform appropriate hosts. The transformed host cells produce recombinant Group B E. tenella proteins which are capable of inducing immunity in chickens to coccidiosis. Antibody prepared against the recombinant protein immunogens is used to isolate and identify the native protein from disrupted E. tenella sporulated oocysts.

公开(公告)号：EP0337589A2

公开(公告)日：1989-10-18

申请号：EP89300328.5

申请日：1989-01-13

申请人： Merck & Co., Inc.

发明人： Liberator, Paul A. ,  Nollstadt, Karl H. ,  Turner, Mervyn J. ,  St. John Crane, Mark ,  Karkhanis, Yashwant D. ,  Chakraborty, Prasanta R. ,  Profous-Juchelka

IPC分类号： C12N15/30 ,  C12P21/02 ,  A61K39/012 ,  C07K13/00

CPC分类号： C07K14/455 ,  A61K39/00 ,  C07K16/20

摘要： Genes coding for novel Group B Eimeria tenella protein immunogens have been isolated and inserted into a novel expression vector which in turn has been used to transform appropriate hosts. The transformed host cells produce recombinant Group B E. tenella proteins which are capable of inducing immunity in chickens to coccidiosis. Antibody prepared against the recombinant protein immunogens is used to isolate and identify the native protein from disrupted E. tenella sporulated oocysts.

摘要翻译： 已经分离了编码新型组B的艾美球虫蛋白免疫原的基因并插入到新的表达载体中，其又被用于转化合适的宿主。 转化的宿主细胞产生能够在鸡中诱导球虫病免疫的重组B组E. tenella蛋白。 使用针对重组蛋白免疫原制备的抗体来分离并鉴定破碎的E.enella孢子化卵囊的天然蛋白质。