公开(公告)号：EP2432884A1

公开(公告)日：2012-03-28

申请号：EP10720510.6

申请日：2010-05-24

申请人： Merial Ltd.

发明人： AUDONNET, Jean-christophe ,  JOLIVET, Edmond

IPC分类号： C12N15/73 ,  C12N15/63 ,  C12N15/68

CPC分类号： C12N15/73 ,  C12N15/635 ,  C12N15/85 ,  C12N2800/107 ,  C12N2800/24 ,  C12P21/00

摘要： The present invention provides a method of maintaining a gram negative bacterium plasmid without the use of antibiotic selection pressure. Further, the invention relates to the drugless plasmids produced including drugless plasmids containing a heterologous gene. The invention also provides formulations and/or compositions comprising the drugless plasmids comprising a heterologous gene, formulations and/or compositions comprising a protein or an immunogen expressed using the drugless plasmids, and methods of administering such formulations and/or compositions to a host. The invention relates to gram negative bacteria containing the drugless plasmids.