公开(公告)号：EP3616717A1

公开(公告)日：2020-03-04

申请号：EP18191922.6

申请日：2018-08-31

申请人： Mikrobiologicky ustav AV CR, v.v.i.

发明人： Sebo, Peter ,  Bumba, Ladislav ,  Espinosa Vinals, Carlos Angel ,  Holubova, Jana ,  Stanek, Ondrej ,  Osicka, Radim ,  Masin, Jiri

IPC分类号： A61K39/10 ,  A61P31/04 ,  C07K14/235

摘要： The present invention provides a polypeptide antigen comprising amino acid sequences derived from adenylate cyclase protein of Bordetella pertussis, comprising (i) an amino acid sequence of a fragment of CyaA, having on its N-terminus an amino acid residue in position between 1132-1166 and having on its C-terminus an amino acid residue in position between 1287-1294, and to its C-terminus fused: (ii) an amino acid sequence of a fragment of CyaA, having on its N-terminus an amino acid residue in position between 1561-1570 and having on its C-terminus an amino acid residue in position between 1680-1681. N-terminally extended derivatives of this sequence are also provided.
Said polypeptide antigen is suitable for use in a method of treatment and/or prevention of a disease caused by Bordetella sp. , in particular Bordetella pertussis, Bordetella parapertussis , or Bordetella bronchiseptica , and in particular against pertussis or whooping cough disease. The invention further provides a chimeric protein, a polynucleotide encoding the polypeptide antigen or chimeric protein, a vector and a host cell for the production of said polypeptide or protein.

公开(公告)号：EP3381931A1

公开(公告)日：2018-10-03

申请号：EP17163566.7

申请日：2017-03-29

申请人： Mikrobiologicky ustav AV CR, v.v.i.

发明人： Masin, Jiri ,  Osicka, Radim ,  Sebo, Peter ,  Osickova, Adriana

IPC分类号： C07K14/235 ,  A61K39/02

CPC分类号： C07K14/235 ,  A61K39/099

摘要： The present invention provides a polypeptide antigen comprising amino acid sequence of adenylate cyclase protein of Bordetella pertussis bearing the following mutations:
(i) substitution of the alanine residue in position 680 by a proline residue, and
(ii) substitution of the valine residue in position 695 by a proline residue,
or one or more fragments thereof, wherein said fragments comprise at least amino acids from position 374 to position 400, from position 500 to position 700, and from position 860 to position 1706.
Said polypeptide antigen is suitable for use in a method of treatment and/or prevention of a disease caused by Bordetella pertussis, in particular against pertussis or whooping cough disease.
The invention further provides a chimeric protein, a polynucleotide encoding the polypeptide antigen or chimeric protein, a vector and a host cell for the production of said polypeptide or protein.