专利检索 ap:("Mixis France S.A.") AND inv:"REYNAUD, Claude-Agnès" 第 1 页

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发明授权
PROCEDE POUR L INDUCTION MAITRISEE DE MUTATIONS SOMATIQUES E T SON APLLICATION EN PROTEOMIQUE 有权
标题翻译：法体细胞突变控制的感应及其用途蛋白质组学

公开(公告)号：EP1463816B1

公开(公告)日：2007-08-29

申请号：EP02804929.4

申请日：2002-12-17

申请人： Mixis France S.A.

发明人： REYNAUD, Claude-Agnès , WEILL, Jean-Claude

IPC分类号： C12N15/10 , C12N15/01 , C07K14/62 , C12N15/90

CPC分类号： C12N15/102 , C12N15/01

摘要： The invention concerns a method for inducing somatic mutations in vitro or ex vivo, comprising (1): adding to the cells to be mutated, in culture conditions and in a medium suited to said cells, of at least a combination of anti-IgM and anti-CD19 and/or anti-DC21 antibodies, while (2) said anti-IgM antibodies are biotinylated and are subjected to specific aggregation by streptavidin coupled with a support. Advantageously, the mutations are controlled and/or adjusted by inhibiting and reactivating and/or stimulating DNA polymerase pol iota. The invention is in particular applicable to rapid and controlled induction of mutations on the BL2 Burkitt's lymphoma.