公开(公告)号：EP0217632B1

公开(公告)日：1996-05-22

申请号：EP86307346.6

申请日：1986-09-24

申请人： Molecular Devices Corporation

发明人： Chow, Calvin Y.H. ,  Lacy, Michael M. ,  Humphries, Gillian M. ,  Parce, John Wallace ,  Kung, Viola T.

IPC分类号： G01N21/25

CPC分类号： G01N21/253 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00621 ,  B01J2219/00659 ,  B01J2219/00702 ,  G01N2201/0438 ,  G01N2201/08

公开(公告)号：EP0217632A3

公开(公告)日：1989-06-07

申请号：EP86307346.6

申请日：1986-09-24

申请人： Molecular Devices Corporation

发明人： Chow, Calvin Y.H. ,  Lacy, Michael M. ,  Humphries, Gillian M. ,  Parce, John Wallace ,  Kung, Viola T.

IPC分类号： G01N21/25

CPC分类号： G01N21/253 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00621 ,  B01J2219/00659 ,  B01J2219/00702 ,  G01N2201/0438 ,  G01N2201/08

摘要： A multibeam photometric measuring device includes a light assembly (12) comprising a single modulated light source along with a filter assembly (19) for passing only a desired band of wavelengths and an optical system for suitably focusing light from the light source. A fiber rotor arrangement (24) is provided for sequentially connecting the collimated light source to each of the plurality of fibers (34) disposed in a distributor (32). Light passing through the wells of the sample plate (40) is captured by discrete photodetector cells (44) disposed on a one-to-one correspondence opposite the wells of the plate. A reference fiber links (52) light from the light source to a separate photodetector (45) to provide a reference light signal with which the sensed photodetector signals are compared. The sensed signals are suitably multiplexed and processed by circuitry which allows control over the accuracy with which the signals are processed on the basis of their instantaneous strengths as well as reference readings taken during system initialization. Enhancement of colour distribution resulting from chromogenic reactions is provided by automatic agitation by mechanism (54) of the reacting agents within the sample wells before a reading is taken at each of the sampling intervals of a kinetic measurement sequence.

公开(公告)号：EP0217632A2

公开(公告)日：1987-04-08

申请号：EP86307346.6

申请日：1986-09-24

申请人： Molecular Devices Corporation

发明人： Chow, Calvin Y.H. ,  Lacy, Michael M. ,  Humphries, Gillian M. ,  Parce, John Wallace ,  Kung, Viola T.

IPC分类号： G01N21/25

CPC分类号： G01N21/253 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00621 ,  B01J2219/00659 ,  B01J2219/00702 ,  G01N2201/0438 ,  G01N2201/08

摘要： A multibeam photometric measuring device includes a light assembly (12) comprising a single modulated light source along with a filter assembly (19) for passing only a desired band of wavelengths and an optical system for suitably focusing light from the light source. A fiber rotor arrangement (24) is provided for sequentially connecting the collimated light source to each of the plurality of fibers (34) disposed in a distributor (32).
Light passing through the wells of the sample plate (40) is captured by discrete photodetector cells (44) disposed on a one-to-one correspondence opposite the wells of the plate. A reference fiber links (52) light from the light source to a separate photodetector (45) to provide a reference light signal with which the sensed photodetector signals are compared. The sensed signals are suitably multiplexed and processed by circuitry which allows control over the accuracy with which the signals are processed on the basis of their instantaneous strengths as well as reference readings taken during system initialization.
Enhancement of colour distribution resulting from chromogenic reactions is provided by automatic agitation by mechanism (54) of the reacting agents within the sample wells before a reading is taken at each of the sampling intervals of a kinetic measurement sequence.

摘要翻译： 一种多光束测光测量装置包括：包括单个调制光源的光组件（12），以及仅用于通过所需波长带的滤波器组件（19）以及用于适当地聚焦来自光源的光的光学系统。 提供光纤转子装置（24）用于顺序地将准直光源连接到布置在分配器（32）中的多根光纤（34）中的每一根。 穿过样品板（40）的孔的光由分立的光电检测器单元（44）捕获，所述单独的光电检测器单元（44）与板的孔相对地一一对应地设置。 参考光纤将来自光源的光链接（52）到单独的光电检测器（45），以提供参考光信号，与所检测的光电检测器信号进行比较。 所感测的信号被适当地多路复用并由电路处理，该电路允许基于其瞬时强度以及在系统初始化期间获取的参考读数来控制处理信号的准确度。 在动力学测量序列的每个采样间隔进行读数之前，通过机构（54）对样品孔内的反应试剂的自动搅动来提供由显色反应导致的颜色分布的增强。