公开(公告)号：EP1421206A2

公开(公告)日：2004-05-26

申请号：EP02759289.8

申请日：2002-08-07

申请人： Molecular Devices Corporation

发明人： KLAUBERT, Dieter ,  DIWU, Zhenjun ,  YI, Guoliang ,  KIRK, Martin

IPC分类号： C12Q1/02

CPC分类号： G01N33/52 ,  Y10S435/975

摘要： The invention encompasses an improved method for measuring membrane potential using compounds of the formula I as petentiometric probes. These probes may be used in combination with other fluorescent indicators such as Indo-1, Fura-2, and Fluo-3, such probes may be used in microplate reading devices such as FLIPRTR, fluorescent imaging plate reader, sold by Molecular Device Corp., of Sunnyvale, CA; flow cytometers; and fluorometers. Such probes are used to measure membrane potential in live cells. X is O. or S. n is 1 or 2.

公开(公告)号：EP1517997A2

公开(公告)日：2005-03-30

申请号：EP03729139.0

申请日：2003-05-23

申请人： MOLECULAR DEVICES CORPORATION

发明人： DIWU, Zhenjun ,  LIAO, Jinfang ,  WANG, Zhiqiang

IPC分类号： C12Q1/37 ,  G01N33/50 ,  A61K38/06 ,  A61K38/07 ,  C09K11/06 ,  C07D311/88

CPC分类号： C07K5/021 ,  C07D311/82 ,  C07D493/10 ,  C07K7/02 ,  C12Q1/37

摘要： Systems, including compositions, kits, and methods, suitable for performing enzyme assays, such as protease assays. The composition may include derivatives of rhodamine 110 that are substitued at the xanthylium nitrogens by a peptide moiety and a morpholine-4-carbonyl moiety, respectively. These compositions may be luminogenic substrates for a large variety of protease enzymes, with utility in a variety of sensitive protease enzyme assays.