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    Improved optical system for a scanning fluorometer
    1.
    发明公开
    Improved optical system for a scanning fluorometer 有权
    Verbessertes optisches Systemfürein abtastendes荧光计

    公开(公告)号：EP0981043A1

    公开(公告)日：2000-02-23

    申请号：EP99116048.2

    申请日：1999-08-16

    申请人： MOLECULAR DEVICES CORPORATION

    发明人： Giebeler, Robert ,  Ogle, David G. ,  Kaye, Roger ,  Hafeman, Dean

    IPC分类号： G01N21/64

    CPC分类号： G01N21/6452 ,  G01N2021/6421 ,  G01N2021/6471

    摘要： A method and apparatus for determining the fluorescence, luminescence, or absorption of a sample is provided. The sample may either be contained within a cuvette or within one or more sample wells within a multi-assay plate. A combination of a broadband source, a monochromator, and a series of optical filters are used to tune the excitation wavelength to a predetermined value within a relatively wide wavelength band. A similar optical configuration is used to tune the detection wavelength. In one aspect, multiple optical fibers are coupled to the excitation source subassembly, thus allowing the system to be quickly converted from one optical configuration to another. For example, the source can be used to illuminate either the top or the bottom of a sample well within a multi-assay plate or to illuminate a single cuvette cell. Similarly, multiple optical fibers are coupled to the detector subassembly. In another aspect, the excitation light and the detected sample emissions pass to and from an optical head assembly via a pair of optical fibers. The optical head assembly is scanned across one axis of the sample multi-assay plate. The multi-assay plate is mounted to a carriage assembly that scans the plate along a second axis orthogonal to the first axis. In another aspect, an optical scanning head assembly is used that includes mirrored optics for coupling the excitation source to the sample and the emitted light to the detector. An elliptical focussing minor is used to magnify and focus the light projected from an optical fiber coupled to the source subassembly onto the sample. The light from the elliptical mirror passes through an aperture in a second elliptical minor prior to impinging upon the sample. The light emitted by the sample within the sample well is reflected by the second elliptical minor and imaged onto the entrance aperture of an optical fiber coupled to the detector subassembly. The optical axes of both minors are slightly offset from the sample well normal. The minor offset minimizes the amount of light reflected from the meniscus of the sample or the bottom surface of the sample well that enters the detection subassembly. In another aspect, stray light reduction is optimized in order to achieve improved sample emission detection uniformity, particularly emission detection uniformity for an array of fluorescence measurements of a specific sample well. In another aspect, time tags are recorded for samples contained within a multi-assay plate.

    摘要翻译： 提供了用于确定样品的荧光，发光或吸收的方法和装置。 样品可以包含在比色皿内或多个测定板内的一个或多个样品孔内。 使用宽带源，单色器和一系列滤光器的组合来将激发波长调谐到相对宽的波长带内的预定值。 使用类似的光学配置来调整检测波长。 在一个方面，多个光纤耦合到激发源子组件，从而允许系统从一个光学配置快速地转换到另一个。 例如，源可以用于在多分析板内照亮样品孔的顶部或底部或照亮单个比色杯池。 类似地，多个光纤耦合到检测器子组件。 在另一方面，激发光和检测到的样品发射经由一对光纤经过光学头组件并从光学头组件传出。 光学头组件沿样品多测定板的一个轴扫描。 多分析板被安装到托架组件，其沿着与第一轴正交的第二轴扫描板。 在另一方面，使用光学扫描头组件，其包括镜像光学器件，用于将激发源耦合到样本，并将发射的光耦合到检测器。 使用椭圆形聚焦小型放大并将从耦合到源子组件的光纤投影的光聚焦到样品上。 来自椭圆镜的光在撞击样品之前穿过第二椭圆形未成形的孔。 由样品阱内的样品发射的光被第二椭圆形小体反射并成像到耦合到检测器子组件的光纤的入口孔上。 两个未成年人的光轴略微偏离样本井正常。 较小的偏移使得从样品的弯液面或进入检测子组件的样品阱的底表面反射的光的量最小化。 在另一方面，为了实现改进的样品发射检测均匀性，特别是特定样品阱的荧光测量阵列的发射检测均匀性，优化了杂散光减少。 在另一方面，记录包含在多测定板中的样品的时间标签。

    Integrated fluid delivery and analysis system
    4.
    发明公开
    Integrated fluid delivery and analysis system 有权
    集成的流体输送和分析系统

    公开(公告)号：EP1231472A1

    公开(公告)日：2002-08-14

    申请号：EP02250913.7

    申请日：2002-02-11

    申请人： Molecular Devices Corporation

    发明人： Giebeler, Robert H. ,  Humphries, Gillian M. ,  Ogle, David G. ,  Hafeman, Dean G. ,  Kaye, Roger A. ,  Modlin, Douglas N. ,  McNerney, Steven A.

    IPC分类号： G01N35/00

    CPC分类号： G01N21/253 ,  G01N35/028 ,  G01N35/1065 ,  G01N35/109 ,  G01N2035/00326 ,  G01N2035/00356 ,  G01N2035/00455 ,  G01N2035/0498 ,  G01N2035/103

    摘要： An integrated fluid delivery and analysis system and components thereof for preparing and/or analyzing samples. The components may include a transport module, a fluidics module, and an analysis module, among others. The system comprises a detection device (1201) configured to detect light from a sample at an examination site, a sample delivery axis extending substantially vertically from the examination site (1604); and at least one material exchange station (1600) along the sample delivery axis above the examination site. Typically, there are: a fluidics head assembly including a dispensing device; a drive mechanism connected to the head assembly for moving the head assembly along a substantially vertical sample delivery axis; and at least two material exchange stations (1600,1602) located along the sample delivery axis, the head assembly being moveable to each material exchange station (1600,1602) to pick up or deposit materials used to conduct an assay.

    摘要翻译： 用于制备和/或分析样品的集成流体输送和分析系统及其部件。 这些组件可以包括运送模块，流控模块和分析模块等。 该系统包括检测装置（1201），检测装置（1201）被配置为检测来自检查部位处的样本的光线，样本递送轴线从检查部位（1604）基本竖直地延伸; 和沿检查部位上方的样本输送轴线的至少一个材料交换站（1600）。 典型地，存在：包括分配装置的流控头组件; 驱动机构，连接到所述头部组件，用于沿着基本竖直的样本传送轴线移动所述头部组件; 和至少两个沿着样本输送轴线定位的材料交换站（1600,1602），头部组件可移动到每个材料交换站（1600,1602）以拾取或沉积用于进行测定的材料。