公开(公告)号：EP2118289B1

公开(公告)日：2013-12-11

申请号：EP08731808.5

申请日：2008-03-10

申请人： Monsanto Technology, LLC

发明人： CALABOTTA, Beth, Jo ,  DEPPERMANN, Kevin, Lee ,  DERSCH, Erik, D. ,  HEISE, Jerald, D. ,  KOESTEL, Angela, Ranae ,  LUDWIG, Cindy, L. ,  MARTINELL, Brian, J. ,  WILLIAMS, Edward

IPC分类号： C12N15/82 ,  A01H5/00 ,  A01H4/00 ,  B02B3/00

CPC分类号： C12N15/8201 ,  A01H4/003 ,  A01H4/008 ,  C12N5/04 ,  C12N9/1029 ,  C12N15/8202 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/8271 ,  C12N15/8274 ,  C12N15/8275 ,  C12N15/8277 ,  C12N15/8281 ,  C12Y203/01081 ,  G01N33/0098

摘要： The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.

公开(公告)号：EP2118289A2

公开(公告)日：2009-11-18

申请号：EP08731808.5

申请日：2008-03-10

申请人： Monsanto Technology, LLC

发明人： CALABOTTA, Beth, Jo ,  DEPPERMANN, Kevin, Lee ,  DERSCH, Erik, D. ,  HEISE, Jerald, D. ,  KOESTEL, Angela, Ranae ,  LUDWIG, Cindy, L. ,  MARTINELL, Brian, J. ,  WILLIAMS, Edward

IPC分类号： C12N15/82 ,  A01H5/00 ,  A01H4/00 ,  B02B3/00

CPC分类号： C12N15/8201 ,  A01H4/003 ,  A01H4/008 ,  C12N5/04 ,  C12N9/1029 ,  C12N15/8202 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/8271 ,  C12N15/8274 ,  C12N15/8275 ,  C12N15/8277 ,  C12N15/8281 ,  C12Y203/01081 ,  G01N33/0098

摘要： The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.