公开(公告)号：EP0185379A2

公开(公告)日：1986-06-25

申请号：EP85116277.6

申请日：1985-12-19

申请人： NABISCO BRANDS, Inc.

发明人： Johnson, Richard A. ,  Antrim, Richard L. ,  Lloyd, Norman E.

IPC分类号： C12N9/92

CPC分类号： C12N9/92 ,  Y10S435/815

摘要： The present invention relates to a process for the production of a purified glucose isomerase which comprises contacting an impure extract containing glucose isomerase and soluble impurities with a weakly basic ion exchange material known to adsorb glucose isomerase; adding a first salt solution of a concentration which removes unadsorbed and weakly adsorbed impurities, but not glucose isomerase; and adding a second, buffered salt solution which elutes the adsorbed glucose isomerase.

公开(公告)号：EP0185379A3

公开(公告)日：1988-05-11

申请号：EP85116277

申请日：1985-12-19

申请人： NABISCO BRANDS, Inc.

发明人： Johnson, Richard A. ,  Antrim, Richard L. ,  Lloyd, Norman E.

IPC分类号： C12N09/92

CPC分类号： C12N9/92 ,  Y10S435/815

摘要： The present invention relates to a process for the production of a purified glucose isomerase which comprises contacting an impure extract containing glucose isomerase and soluble impurities with a weakly basic ion exchange material known to adsorb glucose isomerase; adding a first salt solution of a concentration which removes unadsorbed and weakly adsorbed impurities, but not glucose isomerase; and adding a second, buffered salt solution which elutes the adsorbed glucose isomerase.

公开(公告)号：EP0186871A3

公开(公告)日：1988-05-04

申请号：EP85116276

申请日：1985-12-19

申请人： NABISCO BRANDS, Inc.

发明人： Johnson, Richard A. ,  Antrim, Richard L. ,  Lloyd, Norman E.

IPC分类号： C12N09/92

CPC分类号： C12N9/92 ,  B01D61/145 ,  B01D2313/42 ,  Y10S435/814

摘要： This invention relates to a process for the production of a purified glucose isomerase enzyme which comprises contacting an enzyme extract containing glucose isomerase and impurities with a first polysulfone membrane not normally permeable to glucose isomerase, in the presence of a salt concentration capable of selectively inducing permeation of glucose isomerase through the membrane, and obtaining a glucose isomerase containing permeate.

公开(公告)号：EP0202678A3

公开(公告)日：1988-07-06

申请号：EP86106935

申请日：1986-05-22

申请人： NABISCO BRANDS, INC.

发明人： Lloyd, Norman E. ,  Antrim, Richard L. ,  Johnson, Richard A.

IPC分类号： C12N09/92 ,  C12N09/00

CPC分类号： C12N9/92 ,  C12N9/00 ,  Y10S435/814

摘要： The disclosure is directed to the resolubilization of an insoluble glucose isomerase-amine complex, wherein the amine has the general formula: .
, or
The insoluble enzyme complex may be resolubilized to produce a stable concentrated and purified glucose isomerase preparation by reaction with a esolubilizing mixture comprising a cation exchange resin.