公开(公告)号：EP1602923A4

公开(公告)日：2007-07-25

申请号：EP03786241

申请日：2003-12-24

申请人： NARD KENKYUSHO KK

发明人： KOIKE TOHRU ,  MINAMI NORIO ,  KAWASAKI AKIHIKO

IPC分类号： G01N27/62 ,  C07F3/06 ,  G01N33/68

CPC分类号： G01N33/6818 ,  G01N2458/15 ,  Y10S436/815 ,  Y10T436/16

摘要： A method capable of even with respect to, for example, any biological sample containing multiple compounds, not only confirming the presence of a compound having been converted to a phosphoric acid monoester (peptide, sugar, etc.) among them but also easily identifying the molecular weight thereof; and an additive for mass spectrum measurement which can be used in this method. In this method, use is made of a complex compound exhibiting extremely high coordination capacity for phosphoric acid monoester groups, configured of a single zinc isotope, and multiple mass spectrum data are collected and compared with each other.

公开(公告)号：EP1674857A4

公开(公告)日：2007-08-08

申请号：EP04773797

申请日：2004-10-12

申请人： NARD KENKYUSHO KK

发明人： KOIKE TOHRU ,  KAWASAKI AKIHIKO ,  KOBASHI TATSUHIRO ,  TAKAHAGI MAKOTO

IPC分类号： G01N21/65 ,  C07D213/38 ,  G01N21/27 ,  G01N33/483 ,  G01N33/543

CPC分类号： G01N33/54373 ,  C07D213/38

摘要： The present invention provides a method for measuring a surface plasmon resonance, the method enabling easy detection of the existence of a phosphorylated peptide (protein) and determination whether a peptide is phosphorylated or not in biological materials. The present invention also provides a noble metal compound having high capability of coordination to a phosphorylated peptide, and being conveniently usable in the method. A first method for measuring surface plasmon resonance of the present invention comprises: placing a noble metal compound on a bottom face of a prism, irradiating a light to the prism to detect a reflected light, wherein, the noble metal compound has substituents of following formula (I) on a side opposite to a side contacting the prism, and a subject sample is added to a side having the substituent groups (I) in the noble metal compound. [wherein, X represents a linker group]