公开(公告)号：EP4129050A1

公开(公告)日：2023-02-08

申请号：EP21776678.1

申请日：2021-03-25

申请人： NATIONAL AGRICULTURE AND FOOD RESEARCH ORGANIZATION

发明人： KAWAGISHI, Makiko ,  SASAKI, Kentaro ,  NUNOME, Tsukasa

IPC分类号： A01H1/00 ,  A01H1/02 ,  A01H5/10 ,  C12N15/29

摘要： The present inventors aimed to identify the gene responsible for the temperature-sensitive male sterility trait in "PL12" and to provide a method for producing conditional male sterile plants targeting the gene, and performed whole genome sequence analysis on "PL12" and the original variety thereof to compare the two. As a result, it was found that there was a deletion of about 150 kb in "PL12" chromosome 7. Then, as for this region, six types of partial deletion lines were produced by genome editing, and the presence or absence of the temperature-sensitive male sterility trait in each line was used as an index to successfully narrow the range, containing the responsible mutation, down to about 10 kb. Furthermore, as a result of creating a line with genome editing-disrupted functions of the two genes located in this region, the gene responsible for the temperature-sensitive male sterility trait was successfully identified. Further, it was confirmed that the functions of the gene could be suppressed to impart the above trait to Arabidopsis thaliana and tomato, as in the case of rice.